【摘要】 研究超声波辅助提取菠萝叶中小麦黄素的最佳提取工艺和含量测定。利用单因素实验和正交实验,考察料液比、提取时间、提取温度、超声功率4个因素对提取工艺的的影响;采用高效液相色谱法对小麦黄素含量进行测定。菠萝叶中小麦黄素提取的最佳工艺为m（菠萝叶）∶V（乙酸乙酯）=1.0∶25(g·mL^-1),提取时间90min,超声提取温度75℃,超声功率192W,小麦黄素的平均收率可达0.494%（n=3）。当小麦黄素进样量在0.006～0.12 mg·mL^-1范围内与峰面积分值有良好的线性关系,r=0.99997,加样平均回收率为100.86%（n=5）,相对标准偏差为3.24%。该方法简便有效,结果准确可靠。更多还原

【Abstract】 Studying the optimum methodology of ultrasonic extracting tricin from the leaves of Ananas comosus and determining the content of tricin.By orthogonal experiment and single factor experiment,we investigated the effect of 4 factors containing ratio of solid to liquid,extraction time,extraction temperature,ultrasonic power.After the extraction,the content of tricin was measured by HPLC.The best extraction technology was m（the leaves of Ananas comosus）:V（ethyl acetate）=1.0∶25(g·mL^-1),extraction time was 90 min,extraction temperature was 75℃,ultrasonic power was 192 W,theaverageyield of tricin was 0.494%（n=3）,the amount of tricin had a excellent linear relationship with peak area when the amount of tricin ranged from 0.006～0.120 mg·mL^-1,r=0.99997,average recovery rate was 100.86%（n=5）,relative standard deviation was 3.24%.The method of extraction is feasible and effective,the results are accurate and reliable.更多还原