【摘要】 目的探讨涎腺腺样囊性癌（SACC）患者口腔微生物的多样性和群落差异。方法采集13例SACC患者与10例健康人的唾液样本,提取其口腔菌群的总DNA,经通用引物扩增16srRNA基因,利用高通量测序平台进行细菌16S rRNA基因（V3-V4）区高通量测序分析后,利用Mothur软件分析微生物的多样性和群落结构。结果 SACC组优势种群有16种（相对丰度>1%）,依次是:链球菌属（36.68%）,奈瑟菌属（8.55%）,普雷沃菌属₇（7.53%）,韦荣球菌属（6.37%）等。对照组优势种群有15种,依次有:链球菌属（18.41%）,奈瑟菌属（18.20%）,普雷沃菌属₇（8.89%）,卟啉单胞菌属（6.20%）,梭杆菌属（5.86%）,韦荣球菌属（5.82%）等。2组间有统计学差异的菌门包括厚壁菌门、变形菌门、梭杆菌门（P<0.05）。有统计学差异的菌属包括链球菌属、奈瑟菌属和卟啉单胞菌属（P<0.05）,而嗜二氧化碳噬细胞菌属仅在SACC患者中检出。结论 SACC患者的唾液微生物与健康人群唾液微生物的种群结构存在明显差异。更多还原

【Abstract】 Objective The aim of this study was to identify the differences in microbial diversity and community in patients with salivary adenoid cystic carcinoma（SACC）. Methods Saliva was collected from 13 patients with SACC confirmed by histopathological diagnosis and 10 healthy control subjects. Total metagenomic DNA was extracted. The DNA amplicons of the V3–V4 hypervariable regions of the 16 S rRNA gene were generated and subjected to high-throughput sequencing. Microbial diversity and community structure were analyzed with Mothur software. Results A total of 16 genera of dominant bacteria in the SACC group were found, including Streptococcus（36.68%）, Neisseria（8.55%）, Prevotella₇（7.53%）, and Veillonella（6.37%）, whereas 15 dominant bacteria in the control group were found, including Streptococcus（18.41%）, Neisseria（18.20%）,Prevotella-7（8.89%）, Porphyromonas（6.20%）, Fusobacterium（5.86%） and Veillonella（5.82%）. The statistically different phyla between the two groups were Firmicutes, Proteobacteria and Fusobacterium（P<0.05）. The statistically different genera between the two groups were Streptococcus, Neisseria and Porphyromonas（P<0.05）, and Capnocytophaga was only detected in patients with SACC. Conclusion Significant differences were observed in the oral microorganisms between the two groups.更多还原