【摘要】 建立高效液相色谱法测定食用植物油中没食子酸丙酯、叔丁基对苯二酚、叔丁基对羟基茴香醚、二丁基羟基甲苯、没食子酸、山梨酸6种合成类抗氧化剂的含量。样品经正己烷溶解,再用饱和正己烷的乙腈萃取,振荡,超声25 min,离心后取乙腈层,定容,过0.45μm滤膜。以0.4%乙酸水溶液–乙腈为流动相,流量为0.8 mL/min,梯度洗脱,检测波长为280 nm,采用高效液相色谱法进行检测。6种合成抗氧化剂的质量浓度在0.1～10μg/mL范围内与色谱峰面积呈良好的线性关系,相关系数均大于0.999,方法检出限均为1.0～2.5 mg/kg。样品的加标回收率为79.6%～108.9%,测定结果的相对标准偏差为2.03%～5.77%(n=6)。该方法操作简单,能同时检测6种合成抗氧化剂,适用于多种食用植物油中合成抗氧化剂的测定。更多还原

【Abstract】 A method for the determination of propyl gallate(PG), tertiary butylhydroquinone(TBHQ), butylated hyd-roxylanisole(BHA), butylated hyd-roxytoluene(BHT), gallic acid and sorbic acid in edible vegetable oil by high performance liquid chromatography was established. The samples were dissolved in n-hexane, then extracted with saturated n-hexane acetonitrile, oscillated, ultrasound for 25 minutes, centrifuged, acetonitrile layer was taken, through 0.45 μm?lter membrane after constant volume. The mobile phase was 0.4% acetic acid aqueous solution – acetonitrile, ?ow rate was 0.8 mL/min, gradient elution, detection wavelength was 280 nm, and the detection was carried out by high performance liquid chromatography. The mass concentration of six synthetic antioxidants in the range of 0.1–10 μg/mL showed good linear relationships with the peak area, the correlation coef?cients were more than 0.999, and the detection limits were1.0–2.5 mg/kg. The recoveries of the samples were 79.6%–108.9%, and the relative standard deviations of the results were2.03%–5.77%(n=6). The method is simple and it can simultaneously detect six synthetic antioxidants. It is suitable for the determination of synthetic antioxidants in various edible vegetable oils.更多还原