【摘要】 目的:观察健脾解毒活血汤对幽门螺旋杆菌相关性胃炎(HAG)脾胃湿热证模型小鼠血清白介素-1β(IL-1β)、胃泌素(GAS)及胃内组织Hp感染数的影响,为研制新型抗Hp感染的中药提供实验依据。方法:选取40只SPF级昆明小鼠为实验对象,将其随机分为空白组、模型组、西药组和中药组,每组各10只。采用Hp菌液灌胃以建立HAG脾胃湿热证小鼠模型,西药组予以奥美拉唑钠肠溶胶囊灌胃,中药组予以健脾解毒活血汤灌胃,均连续给药14d。观察比较各组小鼠血清IL-1β、GAS与胃内组织Hp感染情况。结果:模型组小鼠血清IL-1β、GAS的含量较空白组明显升高(P <0. 01,P <0. 05);与模型组比较,中药组和西药组血清IL-1β显著降低(P <0. 05,P <0. 01),GAS水平2组无明显差异。模型组小鼠胃组织Hp感染数较空白组明显增加(P <0. 01),中药组和西药组胃组织Hp感染数较模型组显著降低(P <0. 05)。结论:健脾解毒活血汤对HAG脾胃湿热证模型小鼠血清IL-1β及Hp感染数有明显改善作用,但对GAS影响不大,在一定程度上可有效治疗脾胃湿热型HAG。更多还原

【Abstract】 Objective: To investigate the effect of Jianpi Jiedu Huoxue decoction on the serum levels of interleukin-1β(IL-1β) and gastrin(GAS) and the number of cells with Helicobacter pylori(Hp) infection in gastric tissue in mice with Hp-associated gastritis(HAG) with spleen-stomach damp-heat syndrome,and to provide an experimental basis for the development of new traditional Chinese medicine(TCM) drugs for Hp infection. Methods: A total of 40 specific pathogen-free Kunming mice were selected and randomly divided into blank group,model group,Western medicine group,and TCM group,with 10 mice in each group. A mouse model of HAG with spleen-stomach damp-heat syndrome was established by Hp bacterial solution by gavage. The mice in the Western medicine group were given omeprazole sodium enteric-coated capsules by gavage,and those in the TCM group were given Jianpi Jiedu Huoxue decoction by gavage; both groups were treated for 14 days. The serum levels of IL-1β and GAS and Hp infection in gastric tissue were compared between groups. Results: The model group had significantly higher serum levels of IL-1β and GAS than the blank group(P < 0. 01,P < 0. 05); compared with the model group,the TCM group and the Western medicine group had a significant reduction in the serum level of IL-1β(P < 0. 05,P < 0. 01),while there was no significant difference in the level of GAS. Compared with the blank group,the model group had a significant increase in the number of cells with Hp infection in gastric tissue(P < 0. 01),and compared with the model group,the TCM group and the Western medicine group had a significant reduction in the number of cells with Hp infection in gastric tissue(P < 0. 05). Conclusion: Jianpi Jiedu Huoxue decoction can significantly improve serum IL-1β level and number of cells with Hp infection in mice with HAG with spleen-stomach damp-heat syndrome,with little effect on GAS,and thus exerts a certain therapeutic effect on HAG with spleen-stomach damp-heat syndrome.更多还原