【摘要】 目的:观察健脾扶正方对脾虚湿热证大肠癌术后化疗患者T细胞亚群、Treg细胞及生活质量的影响。方法:将80例脾虚湿热证大肠癌术后患者随机分为治疗组和对照组,每组40例。对照组患者予奥沙利铂~+卡培他滨化疗方案治疗,治疗组患者在化疗治疗期间给予健脾扶正方治疗,共治疗6周。观察两组患者治疗前后中医证候积分、Treg细胞(CD4~+CD25~+Treg、CD4~+FOXP3~+Treg、CD25~+FOXP3~+Treg)、T细胞亚群(CD3~+、CD4~+、CD8~+、CD4~+/CD8~+)、生活质量(KPS评分)的变化,并比较两组临床疗效。结果:治疗组总有效率为90.00%,对照组总有效率为62.50%,治疗组疗效优于对照组(P<0.05);两组患者治疗后中医证候积分均较治疗前降低(P<0.05),且治疗组患者治疗后中医证候积分低于对照组(P<0.05);治疗组患者治疗后CD4~+CD25~+Treg、CD4~+FOXP3~+Treg、CD25~+FOXP3~+Treg水平均明显低于治疗前(P<0.05),治疗组患者治疗后上述指标均低于对照组(P<0.05);治疗组患者治疗后CD3~+、CD4~+、CD4~+/CD8~+水平明显高于治疗前(P<0.05),且治疗组患者治疗后CD3~+、CD4~+、CD4~+/CD8~+水平均高于对照组(P<0.05);治疗组患者治疗后CD8~+水平明显低于治疗前(P<0.05),且治疗组患者治疗后CD8~+水平明显低于对照组(P<0.05);治疗组患者治疗后KPS评分显著高于治疗前(P<0.05),且治疗组患者治疗后KPS评分高于对照组(P<0.05)。结论:健脾扶正方能显著改善脾虚湿热证大肠癌术后化疗患者的临床症状,抑制CD4~+CD25~+Treg、CD4~+FOXP3~+Treg、CD25~+FOXP3~+Treg细胞水平,增强细胞免疫功能,并能改善患者的生活质量。更多还原

【Abstract】 Objective: To observe the effect of Jianpi Fuzheng Recipe on T-cell subsets, Treg cells and quality of life in patients with colorectal cancer of spleen deficiency and damp-heat syndrome after operation.Methods: 80 patients with colorectal cancer of spleen deficiency and damp-heat syndrome were divided into treatment group and control group according to random number table method, with 40 cases in each group. The control group was treated with oxaliplatin and capecitabine chemotherapy regimen, while the treatment group was treated with Jianpi Fuzheng recipe during the period of chemotherapy for 6 weeks. The changes of TCM Treg cells(CD4~+CD25~+Treg, CD4~+FOXP3~+Treg, CD25~+FOXP3~+Treg), T cell subsets(CD3~+, CD4~+, CD8~+, CD4~+/CD8~+),quality of life(KPS scores) before and after treatment were observed and compared between the two groups. The clinical effect of the two groups was compared. Results: The total effective rate was 90.00% in the treatment group and 62.50% in the control group, the curative effect of the treatment group was better than that of the control group(P<0.05). After treatment, the TCIU Symptom scores were all lower than those before treatment(P<0.05), and the scores of TCM syndromes in the treatment group were significantly lower than those in the con-trol group(P<0.05). After treatment, the levels of CD4~+CD25~+Treg, CD4~+FOXP3~+Treg, and CD25~+FOXP3~+Treg in the treatment group were significantly lower than those before treatment(P<0.05); the above indexes in the treatment group were lower than those in the control group(P<0.05); the levels of CD3~+, CD4~+, CD4~+/CD8~+in the treatment group were significantly higher than those before treatment(P <0.05), and the levels of CD3~+, CD4~+,CD4~+/CD8~+in the treatment group were higher than those in the control group(P<0.05). The level of CD8~+in the treatment group was significantly lower than that before treatment(P <0.05), and the level of CD8~+in the treatment group was significantly lower than that in the control group(P<0.05); the KPS score in the treatment group was significantly higher than that before treatment(P<0.05), and the KPS score in the treatment group was higher than that in the control group(P <0.05). Conclusion: Jianpi Fuzheng recipe combined with chemotherapy can significantly improve the clinical symptoms of patients colorectal cancer of spleen deficiency and damp-heat syndrome after operation, inhibit the levels of CD4~+CD25~+Treg, CD4~+FOXP3~+Treg, and CD25~+FOXP3~+Treg cells,and enhance the cellular immune function of the body, and also improve the quality of life of patients.更多还原