【摘要】 目的:探究chemerin对银屑病患者外周血辅助性T淋巴细胞9(helper T cells 9,Th9)/调节性T淋巴细胞(regulatory T cells,Treg)免疫平衡的调节作用及其潜在的机制。方法:选取25例银屑病患者和20例健康志愿者,采用磁珠分离法分离出受试者外周血中CD4+T细胞,行实时RT-PCR和ELISA检测细胞中chemerin和其受体chemR23的表达;用不同浓度的chemerin(50,100,150,200 ng/mL)分别处理从健康志愿者外周血分离得到的CD4+T细胞,行MT T比色法检测细胞活力以筛选最适浓度;采用ELISA检测炎性细胞因子水平;行流式细胞术检测Th9和Treg细胞数。结果:与对照相比,银屑病患者外周血中chemerin和chemR23的表达均显著增加,T h 9/Treg比例明显上升(均P<0.05);选取最适浓度150 ng/mL的chemerin处理CD4+T细胞后,细胞中白细胞介素(IL)-6,IL-9和IL-17的水平均显著增加,Th9/Treg比例明显上升(均P<0.05),而chemR23沉默可反转chemerin对CD4+T细胞的上述作用(均P<0.05)。结论:Chemerin通过chemR23调节银屑病患者外周血CD4+T淋巴细胞中的T h 9/Treg细胞免疫平衡。更多还原

【Abstract】 Objective: To investigate the effects of chemerin on helper T cells 9(Th 9)/regulatory T cells(Treg) in patients with psoriasis and the potential molecular mechanisms.Methods: Twenty-five patients with psoriasis and twenty health y volunteers were selected for th is study. CD4+ T cells were isolated from peripheral blood of samples by magnetic bead separation. Th e levels of chemerin and its receptor chemR23 were detected by real-time RT-PCR and ELISA. CD4+ T cells isolated from the health y volunteers were treated with different concentrations of chemerin(50, 100, 150, 200 ng/mL), then cell viability was detected by MTT assay. Th e expression of inflammatory molecules and Th 9/Treg were detected by ELISA and flow cytometry, respectively.Results: The expressions of chemerin and chemR23 in peripheral blood from patients with psoriasis were higher th an those in health y control(both P<0.05). The Th9/Treg was higher in patients with psoriasis th an th at in health y control(P<0.05). After treating CD4+ T cells with 150 ng/mL of chemerin, the levels of IL-6, IL-9 and IL-17 were increased significantly(all P<0.05). Additionally, Th9/Treg was increased(P<0.05) and the cell balance was disrupt. However, the effects of chemerin on CD4+ T cells were reversed by silencing of chemR23(all P<0.05).Conclusion: Chemerin may regulate the immune balance for Th9/Treg in CD4+ T cells from patients of psoriasis.更多还原