【摘要】 根据笔者实验室前期蛋白质组学研究成果,发现尖孢镰刀菌古巴专化型4号生理小种(Foc4)中的mon1基因影响了尖孢镰刀菌的致病性,为了研究mon1基因在Foc4侵染香蕉过程中的具体作用,利用同源重组方法敲除了Foc4的mon1基因,并对获得的敲除突变体开展表型分析和致病性测定。结果表明,与Foc4野生型菌株相比,敲除突变体生长缓慢、菌丝变细,分支减少及产孢量降低,菌株抵抗外源氧胁迫、细胞壁穿透能力、纤维素利用能力减弱,对巴西蕉苗的致病力明显减弱。由此推测mon1基因在Foc4的生长发育、产孢及致病力等方面具有一定的作用。更多还原

【Abstract】 Based on the clues obtained from our previous proteomics studies, the mon1 gene from Fusarium oxysporum f.sp. cubense race 4(Foc4) was found to play a role in pathogenic infection. To further characterize the functions of the mon1 in the infection of Foc4, the mon1 gene was knocked out through homologous recombination method and the phenotype and pathogenicity of the mutant strains analyzed. The results showed that the mutant strains of Foc4 grew slowly, produced less spores and thinner mycelia with fewer branches, and was lower in their resistance to exogenous oxygen stress, their capacity of cell wall penetration and their cellulose utilization, as compared with the wild strains. Pathogenicity test showed the mutant strains exhibited a significantly low infection against the banana seedlings. All the results suggested that the mon1 gene played a certain role in the growth, development, sporulation and pathogenicity of the Foc4.更多还原