【摘要】 为探求红肉火龙果果实不同组织DNA含量情况,本研究以成熟期红肉火龙果果实为材料,采用改良CTAB法对火龙果果肉、果皮、种子进行总DNA提取。结果表明:红肉火龙果果肉、果皮、种子的OD260/OD280光吸收值分别为1.982 7,1.984 4,1.997 5,均在1.8≤OD260/OD280<2.0,说明DNA纯度均很好。同一红肉火龙果果实不同组织中DNA浓度各不相同,其中种子中的DNA浓度为0.135μg·mL^-1,含量最高,其次果皮中的DNA浓度0.078μg·mL^-1,果肉中DNA浓度最低,为0.036μg·mL^-1。更多还原

【Abstract】 In order to explore the content of DNA in different tissues of red dragon fruit,the total DNA of flesh,pericarp and seed of dragon fruit was extracted by modified CTAB method.The results showed that the OD260/OD280 light absorption values of pulp,peel and seed were 1.982 7,1.984 4 and 1.997 5 respectively,all of which were between 1.8≤OD260/OD280≤2.0,indication that the purity of DNA was very good.The concentration of DNA in different tissues of the same red flesh dragon fruit was different,in which the concentration of DNA in seed was 0.135μg·mL^-1,and the concentration of DNA in pericarp was 0.078μg·mL^-1,the concentration of DNA in pulp was the lowest,0.036μg·mL^-1.更多还原