【摘要】 天然高分子材料丝素蛋白和壳聚糖在一定条件下均具有良好的力学性能和生物相容性,但纯丝素蛋白和纯壳聚糖在其他方面又各有缺陷。采取简单有效的方法将二者进行混合,制备出一种综合性能最优的支架材料,广泛应用于组织工程。首先将蚕茧通过脱胶、溴化锂水溶液溶解、透析、过滤、离心和冷冻干燥等步骤制成再生丝素蛋白;再与经稀醋酸溶解的壳聚糖按一定比例进行物理共混,进而注入自制的模具中冷冻干燥得到初制的丝素蛋白-壳聚糖的复合支架材料。然后将初制的支架材料浸入乙醇中诱导变性,得到最终的丝素蛋白-壳聚糖复合支架材料。通过吸水率测定,采用扫描电镜、万能材料试验机等仪器对改性复合支架材料进行性能研究。结果表明:当丝素蛋白质量含量为40%时,丝素蛋白-壳聚糖共混支架材料的综合性能最佳,是一种很有应用前景的组织工程复合支架材料。更多还原

【Abstract】 The natural macromolecule material silk fibroin and chitosan have good mechanical properties and biocompatibility under certain conditions,but the pure silk fibroin and pure chitosan were defective in other respects.The optimal blending ratio were founded by a simple and effective method.And then the materials were widely used in tissue engineering.First,cocoon were degummed,then lithium bromide aqueous solution dissolved,dialysis,filtration,centrifugation,freeze drying and other steps made of silk fibroin.Second,diluted with chitosan dissolved by acetic acid in a certain proportion for physical blending.Third,the blending solution was poured into the self-made mold and freezedried to obtain the primary silk fibroin-chitosan composite stent material.The primary stent material was immersed in ethanol to induce denaturation to obtain the final silk fibroin-chitosan composite stent material.The scaffolds were characterized by water absorption,scanning electron microscopy(SEM),universal testing machine.The results showed that when the silk fibroin content of 40%,silk fibroin-chitosan scaffold was excellent.These matrices had design potential for tissue engineering.更多还原