【摘要】 目的探讨跨膜蛋白内皮和平滑肌细胞来源的neuropilin样分子（endothelial and smooth muscle cell-derived neuropilin-like molecule,ESDN）与血管内膜増生的关系。方法利用Esdn基因敲除(Esdn^-/-)小鼠及野生型小鼠建立颈总动脉结扎血管内膜增生模型,采用HE染色检测不同时间血管内膜增生情况,计算内膜/中膜（intima/media,I/M）厚度比。Western blot和免疫荧光检测Esdn^-/-小鼠及野生型小鼠颈总动脉结扎不同时间血管平滑肌细胞（vascular smooth muscle cell,VSMC）收缩表型标志物平滑肌α-肌动蛋白（smooth muscleα-actin,SMα-actin）和SM22α以及增殖表型标志物骨桥蛋白（osteopontin,OPN）的表达情况。结果随颈总动脉结扎时间延长,野生型小鼠和Esdn^-/-小鼠I/M厚度比值呈明显升高趋势。结扎术后7 d、14 d和21 d,Esdn^-/-小鼠I/M厚度比值明显高于野生型小鼠;野生型小鼠和Esdn^-/-小鼠颈总动脉壁中VSMC收缩表型标志物SMα-actin、SM22α表达逐渐降低;术后7 d、14 d、21 d,Esdn^-/-小鼠血管组织中SMα-actin、SM22α表达显著低于野生型小鼠;野生型小鼠和Esdn^-/-小鼠颈总动脉壁中增殖型VSMC标志基因OPN表达逐渐升高;术后3 d、7 d、14 d、21 d,Esdn^-/-小鼠血管组织中OPN表达显著高于野生型小鼠。结论 ESDN的表达参与平滑肌细胞表型转化和血管重塑过程。更多还原

【Abstract】 Objective To investigate the correlation of transmembrane protein endothelial and smooth muscle cell-derived neuropilin-like molecule（ESDN） expression and vascular hyperplasia. Methods The intimal hyperplasia model of the Esdn knockout mice and control wild type mice（WT） were prepared by the common carotid artery ligation. HE staining was used to detect the neointima fromation at different time points. Western blot was used to detect the expression of smooth muscle α-actin（SM α-actin）, SM22α and osteopontin（OPN）, the phenotype marker genes in vascular smooth muscle cells. Immunofluorescence was used to detect the SM α-actin protein expression and distribution in aorta. Results At 7, 14 and 21 days after arterial ligation, the area of the intimal hyperplasia and the I/M ratio were significantly increased in the Esdn^-/- arteries compared with WT control arteries. The expression of SM α-actin and SM22α were decreased. However, the expression of OPN, the synthetic marker gene, was increased in the Esdn^-/- arteries at 3, 7, 14 and 21 days after arterial ligation. Furthermore, the expression of SM α-actin was enhanced in the media area of aorta in Esdn^-/- arteries using immunofluorescence staining. Conclusion The expression of ESDN may be involved in vascular smooth muscle cell phenotypic switching and vascular remodeling.更多还原