【摘要】 以不同生长期木枣(Ziziphus jujube Mill.)果实为材料,测定果实纤维素酶(Cx-cellulase)、β-半乳糖苷酶(β-Gal)、果胶甲酯酶(PME)和多聚半乳糖醛酸酶(PG)等4种细胞壁酶的活性,探究其与木枣果实生长发育的相关性。结果表明,在整个生长过程中,Cx-cellulas酶活性相对平稳上升,β-Gal酶活性从全红期到完熟期迅速达到最大值,PME活性从半红期到全红期迅速下降;PG酶活性在完熟期明显升高;推测Cx-cellulase与木枣果实膨大相关,是木枣软化的重要物质之一;PME在启动木枣果实的转色中起作用;β-Gal和PG则在木枣果实成熟期起关键作用。更多还原

【Abstract】 Using the fruit of different growing period of jujube(Ziziphus jujube Mill.) as materials,the activity of cell wall enzymes of cellulase(Cx-cellulase), β-galactosidase(β-Gal), pectin methylesterase(PME), and polygalacturonase(PG) were observed and discussed the related to the jujube fruit growth processes. The results showed that : in the whole growth, Cx-cellulase was rised stablely, β-Gal reached maximum from full red stage to full ripe stage, PME reduced from half red stage to full red stage, and PG was rised in full ripe stage. It was speculated that Cx-cellulase was related to fruit enlargement of jujube, and it is one of the important substances of softening of jujube fruit. PME may be played a role in starting discolour, β-Gal and PG play a key role in the mature period of jujube fruit.更多还原