【摘要】 研究黄瓜离体雌核发育早期相关基因的表达,为进一步大幅提高黄瓜未受精子房培养胚胎诱导率奠定理论基础。采用基因表达谱芯片和qPCR技术,提取黄瓜未受精子房培养早期胚珠部位总RNA,从转录组水平分析了高频基因型和低频基因型黄瓜未受精子房培养早期差异基因表达。筛选出与胚胎发育相关的基因47个,经qPCR验证,获得8个候选基因,在高频型材料培养的前6 d均呈现高水平表达,在低频型材料培养中呈现低水平表达或总体变化下降的趋势。其中3个基因与响应水杨酸、茉莉酸防御系统,响应脱落酸刺激,生长素诱导结合蛋白以及玉米素合成途径中细胞分裂素脱氢酶的表达相关;2个基因参与POD酶活性表达;3个基因分别与苏氨酸-丝氨酸蛋白激酶、水通道蛋白以及机械敏感性离子通道蛋白相关。初步认为上述8个基因是与黄瓜离体雌核发育早期过程相关的重要基因。更多还原

【Abstract】 Differentially expressed genes of cucumber in vitro gynogenesis in the early stage were analyzed by comparing the transcriptome of early cultured ovaries from both high and low regeneration frequency genotypes with the method of gene chips and qPCR technology. The results showed that 8 differentially expressed genes we selected, which appeared high level expressions in high regeneration frequency genotype, but low level expressions or overall decline in the low regeneration frequency genotype from 0 th to 6 th day of culture. 3 genes were found to be associated with hormonal stimulation response: respectively response to salicylic acid and jasmonic acid defense system, abscisic acid stimulation, auxin-induced binding protein and CTK expression in the Zeatin biosynthesis. 2 genes were found to be involved in the expression of POD activity which played an important role in the progress of biological oxidation. The remaining 3 genes were related to STK activity, aquaporin, mechanosensitive ion channel protein and separately participated in response to environmental stress, response to borates, arsenites and other boron, arsenic materials, transmit signals inside and outside cells and other biological processes.It is preliminarily believed that the 8 genes were important during the early stage of cucumber in vitro gynogenesis.更多还原