【摘要】 目的初探黑蒜对急性酒精性肝损伤的保护作用及其机制。方法小鼠分5组,用高和低浓度黑蒜溶液、生理盐水(阴性对照)、水飞蓟素片溶液(阳性对照)分别灌胃一周,采用50%酒精灌胃的方法构建小鼠的急性酒精性肝损伤模型,另有一组小鼠以生理盐水灌胃一周后不建立模型,为空白对照。各组小鼠取血清测定ALT、SOD活性及取肝切片观察组织学改变,将实验组与各对照组小鼠对比,以初探黑蒜对急性酒精性肝损伤的保护作用及其机制。结果阴性对照与空白对照相比,小鼠血清ALT水平显著升高(P≤0.01),肝切片见明显组织损伤,SOD活性明显降低(P≤0.05)。高剂量黑蒜实验组的血清ALT较阴性组有显著降低(P≤0.01),肝切片可见组织损伤程度显著降低,SOD活性明显升高(P≤0.05)。结论 50%酒精灌胃可建立小鼠急性酒精性肝损伤模型,高浓度黑蒜通过抗氧化对小鼠急性酒精性肝损伤发挥保护作用。更多还原

【Abstract】 OBJECTIVE To explore whether black garlic has preventive and protective effect against acute alcoholic hepatic injury and its mechanism,our group conducted an experiment in vivo.METHODS Mice were divided into 5 groups(blank control group,negative control group,positive control group,black garlic at low and high concentration groups) and gavaged for 7 days.50% ethyl alcohol was chosen to set up model of acute hepatic injury.Content of ALT and T-SOD in blood of ice along with histological sections in each group were measured and analysed.RESULTS Compared with blank control group without alcohol,negative control group showed significant rise in ALT(P≤0.01)and sharp drop in SOD(P≤0.05).Compared with negative control group,which gavaged with normal saline,black garlic at high concentration could reduce the ALT significantly(P≤0.01).Histological sections showed the same result.CONCLUSION 50% ethyl alcohol can set up the model of acute hepatic injury in mice;black garlic at high concentration has protective effect against acute alcoholic hepatic injury through antioxidation.更多还原