【摘要】 目的:建立香苓开胃方中广藿香酮含量的HPLC测定方法。方法:采用高效液相色谱法（HPLC）测定香苓开胃方水煎剂中广藿香酮的含量,色谱柱Extend-C₁₈（4.6mm×250mm,5μm）,流动相为乙腈（A）-0.2%的磷酸水溶液（B）,梯度洗脱（0～20min,A 55%～60%）,进样量:10μL,流速:1mL·min^-1。检测波长为310nm,柱温为30℃。结果:广藿香酮在0.00798～0.66500μg（r=1.0000）范围内线性关系良好;平均回收率（N=6）为108.58%,RSD为3.24%。香苓开胃方中广藿香酮含量范围为0.339～1.408 mg·dose^-1。结论:本实验方法简便准确,回收率、重复性均能达到要求,可用于香苓开胃方的质量控制。更多还原

【Abstract】 Object To establish a method for determination of patchoulenone in Xiang Ling appetizing prescription by High Performance Liquid Chromatography（HPLC）. Methods: The content of Patchoulenone from the water extract of Xiang Ling appetizing prescription was detected by HPLC. The Extend-C₁₈（4.6 mm×250 mm,5μm）column was used for chromatography. The mobile phase consisted of acetonitrile（A）-0.2%phosphoric（B） with solution gradient elute（0～20 min,A 55%～60%）, injection volume was 10 μL, flow velocity was 1.0 mL·min^-1, wave length was 310 nm,column temperature was set at 30 ℃. Results: In the range of 0.00798～0.66500μg（r=1.0000）, Patchoulenone has a good linear regression, and average recovery was 108.58% with RSD3.24%（N =6）. Purity of Patchoulenonein Xiang Ling appetizing prescription is 0.339～1.408 mg·dose^-1. Conclusion: This method is simple and accurate, the recovery rate and repeatability can meet the requirements, and can be used for the quality control of Xiang Ling appetizing prescription.更多还原