【摘要】 目的探讨咯利普兰对大鼠脊髓缺血再灌注损伤(SCII)后神经运动功能及氧自由基的影响。方法将60只SD大鼠随机分为假手术组(Sham组)、SCII组及咯利普兰组,每组20只。SCII组及咯利普兰组建立大鼠SCII模型,Sham组仅于大鼠胸主动脉置管,不阻断血流。建模前30 min至实验结束,给予咯利普兰组大鼠腹腔注射咯利普兰,Sham组和SCII组注射相应体积的二甲基亚砜。于建模后6 h、12 h、24 h及48 h,每组分别取5只大鼠,进行体感诱发电位检测及后肢神经运动功能(BBB)评分,检测大鼠脊髓组织中超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量。结果术后各时点,Sham组、咯利普兰组、SCII组BBB评分依次降低,体感诱发电位潜伏期依次延长而波幅依次减小,脊髓组织中SOD活力依次降低而MDA含量依次升高(均P <0. 05)。结论咯利普兰可减少大鼠SCII后氧自由基的生成,从而保护神经运动功能。更多还原

【Abstract】 Objective To investigate the effect of rolipram on neuromotor function and oxygen free radicals after spinal cord ischemia reperfusion injury( SCII) in rats. Methods Sixty SD rats were randomly divided into sham-operation group( Sham group),SCII group,or rolipram group,with 20 rats in each group. SCII rat model was established in the SCII group and the rolipram group,while thoracic aorta catheterization without blood flow blocking was conducted in the rats of the Sham group. Intraperitoneal injection of rolipram was performed in the rolipram group30 min before modeling till the experiment was finished,while Sham group and SCII group were injected with corresponding volume of dimethyl sulfoxide. At 6 h,12 h,24 h and 48 h after modeling,5 rats was selected in each group,then the somatosensory-evoked potential detection and Basso,Beattie and Bresnahan( BBB) scoring of the hind-limb were performed,and the superoxide dismutase( SOD) activity and malondialdehyde( MDA) content of rats spinal tissues were detected. Results At each time point of post-operation,the BBB score decreased; latent period of the somatosensory-evoked potential prolonged,while the amplitude decreased; the SOD activity of the spinal tissues decreased,while MDA content increased in the order of Sham group,rolipram group and SCII group( all P < 0. 05).Conclusion Rolipram can reduce the oxygen free radicals production after spinal cord ischemia reperfusion injury in rats,and thereby can protect the neuromotor function.更多还原