【摘要】 目的:观察阿米洛利对小鼠损伤足细胞尿激酶受体(uPAR)表达的影响及其降蛋白尿作用,探讨其降低蛋白尿的作用机制。方法:分别构建脂多糖(LPS)诱导足细胞损伤的细胞模型和动物模型,实验分为3组:正常对照组、LPS组、LPS+阿米洛利组。采用考马斯亮蓝法检测动物模型各组24 h尿蛋白,同时采用转板迁移实验检测足细胞活动力,采用免疫荧光和实时荧光定量PCR分别检测细胞模型和动物模型上足细胞uPAR蛋白和uPAR mRNA的表达情况。结果:小鼠24 h蛋白尿检测结果显示, LPS+阿米洛利组与正常对照组均低于LPS组(均P<0.05);在转板迁移实验中,LPS+阿米洛利组与正常对照组每个视野足细胞数低于LPS组(均P<0.05);LPS组足细胞uPAR蛋白和uPAR mRNA表达均明显高于正常对照组与LPS+阿米洛利组(均P<0.05)。结论:阿米洛利可能通过抑制受损足细胞uPAR的表达,降低足细胞活动力,从而起到降蛋白尿的作用。更多还原

【Abstract】 Objective:To observe the effect of amiloride on the expression of urokinase plasminogen activatorreceptor(uPAR) in the injured podocytes and its effect on proteinuria.Methods:The lipopolysaccharide(LPS) mouse model of transient proteinuria and the injured podocytes model were established. The mice or cells were divided into normal control group, LPSgroup and LPS+amiloride group. The 24 h urine protein was detected by Coomassie brilliant blue method. The podocyte activity was detected by plate migration assay, and the expression of uPAR protein and mRNA in podocytes was detected by immunofluorescence and real-time fluorescence quantitative PCR, respectively. Results:Compared with LPS group, the 24 h urine protein level, the number of podocytes and the expression of uPAR mRNA and protein in LPS+amiloride group were significantly reduced(P<0.05).Conclusion:Amiloride could reduce proteinuria in the injured podocytes through down-regulating the expression of uPAR and decreasing the activity of podocytes.更多还原