【摘要】 目的:观察表没食子儿茶素没食子酸酯（EGCG）对黄曲霉毒素B₁（AFB₁）致人正常肝细胞（HL7702细胞）氧化损伤的影响。方法:将HL7702细胞分为正常对照组、溶剂对照组（0.3%DMSO）、AFB₁染毒组和低、中、高剂量EGCG组（5μg/mL、10μg/mL、20μg/mL）。AFB₁染毒组用60μg/mL AFB₁作用细胞24 h,建立肝HL7702细胞氧化应激模型。低、中、高剂量EGCG组预先用相应浓度EGCG处理细胞1 h,再加入60μg/mL AFB₁作用24 h。用CCK-8法检测细胞存活率,DCFH-DA荧光探针法检测细胞内活性氧（ROS）,并检测细胞培养上清液中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、乳酸脱氢酶（LDH）以及受损细胞内超氧化物歧化酶（SOD）、丙二醛（MDA）的含量。结果:与对照组相比,AFB₁染毒组ALT、AST、MDA及ROS水平明显升高,肝细胞存活率、SOD酶活性明显降低,差异均有统计学意义（均P<0.05）;与AFB₁染毒组相比,EGCG处理可使ALT、AST、MDA及ROS水平明显降低,肝细胞存活率、SOD活性明显升高（均P<0.05）。结论:EGCG可明显减轻AFB₁诱导的肝细胞损伤,其作用机制可能与抗氧化损伤有关。更多还原

【Abstract】 Objective:To study the effect of epigallocatechin gallate（EGCG） on human hepatic cell line（HL7702） damaged by aflatoxin B₁（AFB₁）.Methods:HL7702 cells were divided into control group,solvent control group（0.3% DMSO）,AFB₁ group,and low-,medium-,and high-dose（5,10 and 20 μg/mL） of EGCG groups.The cells in AFB₁ group and EGCG groups were treated with 60 μg/mL AFB₁ for 24 h.EGCG groups cells were treated with different concentrations of EGCG at 1 h before the addition of AFB₁.CCK-8 assay was used to detect cell viability.The reactive oxygen species（ROS） in cells was measured by DCFH-DA fluorescence probe.The contents of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,and lactate dehydrogenase（LDH） in cells supernatant as well as the intracellular superoxide dismutase（SOD） and malondialdehyde（MDA） in cells were determined.Results:Compared with the control group,the levels of ALT,AST,MDA and ROS in AFB₁ group were significantly increased,while the hepatocytes survival rate and the activity of SOD were decreased（P<0.05）.All these effects of AFB₁ were reversed by pretreatment with EGCG（P<0.05）.Conclusion:EGCG could reduce hepatocytes injury induced by AFB₁,and the mechanism might be related to antioxidant damage.更多还原