【摘要】 针对目前凝血检测仪器存在的成本昂贵操作复杂等问题,制作了一种基于丝网印刷技术的多壁碳纳米管(MWCNTs)增强型电化学传感器用于凝血酶原时间(PT)测量。利用凝血酶切割凝血酶底物实验验证了计时电流法检测凝血酶原时间原理的可行性。接着对血浆凝血酶原时间参数进行测量,并用SYSMEX CS 5100光学凝血仪验证测量结果。经过测试,凝血酶验证性实验中MWCNT型电化学传感器的电流响应强度较普通电化学传感器增加了(36±1)%,重复实验出峰时间和峰值电流变异系数分别为2.99%和3.27%。测试不同血样PT值,能够清晰的显示出区分度,且挑选三组血样进行PT重复实验,出峰时间变异系数分别为2.26%、3.22%和2.96%,实验结果与医院的临床结果线性拟合决定系数R~2为0.986。MWCNTs增强型型电化学传感器用于PT测试具有良好的重复性和一致性,易于批量生产,大大降低了凝血测量的成本,且适合多种场合的测量,在即时检测领域具有极大潜力。更多还原

【Abstract】 The determination of coagulation parameters is of crucial significance in the guided treatment of anticoagulant drug users and patients with liver diseases.Considering the challenges associated with commercial coagulation detection instruments such as high cost and complex operation,a MWCNTenhanced screen-printed electrochemical sensor was developed for coagulation measurement.Initially,the measurement of thrombin substrate cleaved by thrombin was used to evaluate the feasibility of prothrombin time(PT)detection by chronoamperometry.Then the plasma PT parameters were measured and the results were validated using the SYSMEX CS 5100 optical coagulator.The response intensity of a MWCNT electrochemical sensor in thrombin validation experiments increase by(36±1)% compared with a general electrochemical sensor and the peak time coefficient of variation and peak current coefficients of variation are 2.99% and 3.27% respectively.The testing of PT values of different blood samples clearly shows discrimination.Three groups of blood samples are selected for repeated PT parameter measurements and the coefficients of variation are 2.26%,3.22%,and 2.96%respectively.The linear fitting decision coefficient R~2 is 0.986 for clinical results.The MWCNT screen-printed electrochemical sensor for PT testing has good repeatability and consistency,is easy to mass produce,reduces the cost of coagulation measurement,and is suitable for measurement in many circumstances.As such,this sensor has great potential in the field of point-of-care testing.更多还原