【摘要】 建立超临界流体色谱（SFC）分离纯化补骨脂中异补骨脂素和补骨脂素的工艺方法。采用YMC-C₁₈、YMC-Diol和YMC-NH₂等3种色谱柱,甲醇、乙腈、乙醇、异丙醇等4种改性剂,流动相流速2～6 mL×min^-1、温度300～325 K、压力10～14 MPa条件下,考察了异补骨脂素和补骨脂素的纯化效果。放大得到制备型SFC条件为:色谱柱YMC-Diol;流动相为含有j=2%甲醇的超临界二氧化碳,流速14.0 mL×min^-1;分离温度313 K;压力12 MPa。结果表明,分离得到的异补骨脂素和补骨脂素的纯度均大于98%,由Van’t Hoff曲线可知实验条件下SFC分离过程为焓控过程。更多还原

【Abstract】 A supercritical fluid chromatography（SFC） method was established for the separation and purification of isopsoralen and psoralen from Psoralea corylifolia L. The analytical conditions were: three columns including YMC-C18, YMC-Diol and YMC-NH₂, four modifiers including methanol, acetonitrile, ethanol and isopropanol, flow rate of mobile phase 2～6 m L×min^-1, separation temperature 300～325 K and separation pressure 10～14 MPa. Moreover, the conditions for preparative SFC were: column = YMC-Diol, mobile phase = supercritical carbon dioxide containing 2%（v/v） methanol at a flow rate of 14.0 mL×min-1, separation temperature = 313 K and separation pressure = 12 MPa. The result shows that the purities of isopsoralen and psoralen are both above 98%. The Van’t Hoff curve shows that the SFC separation is an enthalpy control process under the experimental conditions.更多还原