【摘要】 目的探讨甲状腺癌患者血清中半乳糖血凝素-1(Gal-1)、紧密连接蛋白-1(Cla-1)水平测定对甲状腺肿瘤病情评估的价值。方法收集该院收治的甲状腺肿瘤患者104例,其中甲状腺乳头癌32例,甲状腺滤泡癌18例,甲状腺腺瘤54例;另选取20例甲状腺功能各指标、甲状腺彩超均正常的志愿者作为对照组。采用酶联免疫吸附试验(ELISA)检测血清Gal-1、Cla-1水平,采用qRT-PCR和Western bolt检测组织Gal-1、Cla-1mRNA和蛋白表达情况,qRT-PCR检测肿瘤组织细胞增殖促进基因、肿瘤侵袭基因mRNA的表达量。采用Pearson相关分析甲状腺癌患者血清Gal-1和Cla-1与细胞增殖、肿瘤侵袭基因mRNA表达的相关关系。结果 4组研究对象性别差异有统计学意义(P<0.05),4组血清Gal-1、Cla-1水平差异均有统计学意义(P<0.05),其中甲状腺乳头癌组血清Gal-1和Cla-1水平均高于其余3组;甲状腺滤泡癌组血清Gal-1和Cla-1水平均高于甲状腺腺瘤组和对照组,差异有统计学意义(P<0.05);与对照组相比,甲状腺腺瘤组Gal-1水平显著升高(P<0.05)。与对照组相比,甲状腺乳头癌组、甲状腺滤泡癌组、甲状腺腺瘤组的组织中Gal-1、Cla-1mRNA和蛋白表达量均显著降低(P<0.05)。甲状腺癌患者肿瘤组织细胞增殖促进基因EDAG-1和Survivin mRNA表达量均高于对照组,CCNG2mRNA表达量均低于对照组(P<0.05);肿瘤侵袭基因STAT3和FAK mRNA表达量高于对照组,Beclin-1mRNA表达量均低于对照组(P<0.05)。血清Gal-1与EDAG-1、Survivin、FAK呈明显正相关关系,血清Cla-1与Survivin、STAT3、FAK呈明显正相关关系;两者均与CCNG2、Beclin-1呈负相关关系。结论 Gal-1、Cla-1有望成为血清标志物,为甲状腺癌早期诊断和筛查提供依据。更多还原

【Abstract】 Objective To investigate the determination value of serum Galectin-1(Gal-1)and Claudin-1(Cla-1)level for assessing tumor severity in patients with thyroid cancer.Methods Totally 104 cases of thyroid tumors were collected,including 32 cases of papillary thyroid cancer,18 cases of follicular thyroid cancer and 54 cases of thyroid adenoma.Another 20 volunteers with normal thyroid function and color Doppler ultrasound were selected as control group.The serum levels of Gal-1 and Cla-1 were detected by ELISA,the expression of Gal-1 and Cla-1 in tissues were detected by qRT-PCR and Western bolt,and the expression of proliferation-promoting genes and invasive genes in tumor tissues were detected by qRT-PCR.Pearson was used to analyze the correlation of serum Gal-1 and Cla-1 with cell proliferation and expression of invasive gene mRNA in patients with thyroid cancer.Results There were significant differences in serum Gal-1 and Cla-1 levels between the four groups(P<0.05).There were significant differences in serum Gal-1 and Cla-1 levels among the four groups(P<0.05),in which the serum Gal-1 and Cla-1 levels of thyroid papillary cancer were higher than those of the other three groups;the serum Gal-1 and Cla-1 levels of thyroid follicular cancer were higher than those of thyroid adenoma and control group(P<0.05);the serum Gal-1 levels of thyroid adenoma were significantly higher than those of control group(P<0.05).Compared with the control group,the expressions of Gal-1 and Cla-1 in papillary thyroid cancer,follicular thyroid cancer and thyroid adenoma were significantly decreased(P<0.05).Compared with the control group,the expressions of Gal-1 and Cla-1 in papillary thyroid cancer,follicular thyroid cancer and thyroid adenoma were significantly decreased(P<0.05).The expression of EDAG-1 and Survivin in thyroid cancer tissue was higher than that in control group,and the expression of CCNG2 was lower than that in control group(P<0.05).The expression of STAT3 and FAK in thyroid cancer tissue was higher than that in control group,and the expression of Beclin-1 was lower than that in control group(P<0.05).Serum Gal-1 was positively correlated with EDAG-1,Survivin and FAK,while serum Cla-1 was positively correlated with Survivin,STAT3 and FAK;both were negatively correlated with CNCG2 and Beclin-1.Conclusion Gal-1 and Cla-1 are expected to become serum markers for the early diagnosis and screening of thyroid cancer.更多还原