【摘要】 目的通过检测乙型肝炎病毒相关性肾炎(HBV-GN)患者血清中的自身抗体,分析自身抗体在HBV-GN中的诊断和预测价值,从而为该病的防治提供新的思路。方法对23例HBV-GN和25例乙型肝炎非肾炎患者进行病例分析,并采用间接免疫荧光法和免疫印迹法分别检测患者血清抗核抗体(ANA)和抗可溶性核抗原(ENA)抗体,比较每组抗核抗体的阳性率和核型以及抗ENA抗体谱的阳性率和种类,并对乙型肝炎非肾炎患者进行随访。结果 HBV-GN患者抗核抗体和抗ENA抗体谱阳性率显著增高,其中ANA核型以颗粒型为主,抗ENA抗体谱中Ro-52和AMA-M2居多,且自身抗体阳性的乙肝患者更易发生HBV-GN。结论自身抗体在乙型肝炎病毒相关性肾炎中有一定的检测价值,可以为疾病的预测和早期诊断提供新的参考指标。更多还原

【Abstract】 Objective To provide a new method for prevention and treatment of hepatitis B virus associated glomerulonephritis(HBV-GN)through detecting the serum autoantibodies in patients with HBV-GN and analyzing the diagnostic and predictive value of autoantibodies in HBV-GN.Methods Case analysis was performed on 23 patients with HBV-GN and 25 HBV patients without nephritis,and indirect immunofluorescence and immunoblotting were used respectively to detect antinuclear antibodies(ANA)and anti extractable nuclear antigen(ENA)antibody in serum,then the positive rate and karyotype of ANA as well as the positive rate and type of anti ENA antibody were compared in each group,then follow-up was conducted in hepatitis b patients without nephritis.Results The positive rate of ANA and anti ENA antibody in patients with HBV-GN increased significantly,and the karyotype of ANA was mainly granular pattern,and there were more Ro-52 and AMA-M2 in anti ENA antibody,and HBV patients with positive autoantibodies were more likely to develop into HBV-GN.Conclusion Autoantibodies have a certain value in HBV-GN,which can provide new reference for the prediction and early diagnosis of the diseases.更多还原