【摘要】 目的:建立射干扶正口服液的指纹图谱。方法:采用高效液相色谱法,色谱柱为Eclipse Plus C₁₈柱（250 mm×4.6 mm,5μm）;流动相为乙腈-1%甲酸水溶液梯度洗脱;流速:1 m L/min;检测波长:242 nm;柱温:30℃;进样量:20μL。采用中药指纹图谱相似度评价系统对10批射干扶正口服液指纹图谱进行评价。结果:标定了20个共有峰,其中4个共有峰分别为车前子苷、射干苷、黄芪甲苷、大黄素。10批射干扶正口服液指纹图谱相似度均大于0.971。结论:该法稳定、快速、简便,结果准确、可靠,可用于射干扶正口服液的质量控制。更多还原

【Abstract】 Objective:To build the fingerprint of SheGan FuZheng oral liquid. Methods: Using HPLC method,chromatographic column was Eclipse Plus C₁₈ column（250 mm×4.6 mm, 5 μm）; mobile phase was acetonitrile-1%formic acid aqueous solution as gradient elution; flow rate: 1 mL/min; detection wavelength: 242 nm; column temperature: 30℃; sample size: 20 μL. Fingerprints of ten batches of SheGan FuZheng oral liquids were evaluated using similarity evaluation system for herbal fingerprint. Results: All 20 common peaks were marked, among them,four common peaks were plantaridin, belamcandin, astragaloside A and emodin respectively. Fingerprint similarity of ten batches of SheGan FuZheng oral liquids were greater than 0.971. Conclusion: The method, stable, rapid,simple and convenient, accurate and reliable results, could used for quality control of SheGan FuZheng oral liquid.更多还原