【摘要】 在冬季的松花江底泥中筛选出4株高效耐低温菌,并对10℃下脱氮效率最高的33号菌进行了表型鉴定和遗传学鉴定。考察了温度、pH值和常用碳源对该菌株在低温下脱氮效果的影响,并对该菌株的低温异养硝化-好氧反硝化性能进行了研究。结果表明:该低温硝化菌株为Pseudomonas菌属,命名为Pseudomonas sp. M-33。该菌株能在2～35℃下生长并对氨氮进行去除,适宜在中性偏碱的条件下生长,能够在低温下利用柠檬酸钠、醋酸钠、丙三醇和葡萄糖等碳源。该菌株在10℃、24 h内对氨氮的去除率达到90%以上且无亚硝酸盐氮积累。M-33菌株可在好氧状态下对硝酸盐氮和亚硝酸盐氮进行反硝化去除,在硝酸盐氮反硝化过程中会产生一定的亚硝酸盐氮积累并逐渐去除。更多还原

【Abstract】 Four cold-resistant strains were isolated from the sediment of the Songhua River in winter. Phenotype analysis and genetic identification were conducted on No. 33 strain which showed the highest nitrogen removal efficiency under 10 ℃ .Effects of temperature,p H,and carbon sources on the nitrogen removal performance of No. 33 strain were investigated. The performance of heterotrophic nitrification-aerobic denitrification of No. 33 strain at low temperature were studied as well. The results showed that this low temperature nitrifying strain was Pseudomonas sp.,named Pseudomonas sp. M-33.M-33 strain could grow and remove ammonia nitrogen under temperature of 2-35 ℃,and preferred slightly alkaline environment. M-33 could utilize sodium citrate,sodium acetate,glycerol,and glucose as carbon sources at low temperature. During the heterotrophic nitrification process,M-33 removed more than 90% of ammonia nitrogen at 10 ℃ in 24 hours without nitrite accumulation. A certain amount of nitrite was accumulated during the denitrifying of nitrate,which was gradually degraded.更多还原