【摘要】 迅速发展的集约化畜禽养殖场产生了大量的厌氧消化液(ADE),若不妥善处置将对环境产生不可逆的破坏。在光生物反应器(PBR)内,微藻可以利用ADE丰富的无机盐满足自身生长的同时实现废水的处理。为此,构建了菌藻共生体系(进水为未灭菌ADE)和单一微藻体系(进水为灭菌ADE)两个PBR,对比分析了其对ADE的处理效能及微藻生物量的积累情况。结果表明,相比单一微藻体系,菌藻共生体系可显著提高对ADE的处理效能,对TN、TP和COD的去除率分别高达(82. 1±1. 9)%、100%和(83. 5±1. 8)%。三维荧光光谱分析表明,灭菌使得ADE废水中荧光类有机物的成分和峰值均发生了变化;相较于单一微藻体系,菌藻共生体系对荧光类有机物的降解优势明显,大分子蛋白类有机物被有效降解成小分子腐殖酸类有机物。菌藻共生体系可应用于扩大化培养微藻同步实现ADE废水的处理,该体系节约了高温高压灭菌的成本,并可获得更为理想的ADE处理效果。更多还原

【Abstract】 A large amount of anaerobic digestion effluent( ADE) has been produced as the rapid development of intensive livestock and poultry farms,and irreversible damage will be imposed to the environment if ADE is not properly disposed. Microalgae can consume abundant nutrient in ADE to meet their own growth and achieve ADE treatment in the photobioreactor( PBR). Therefore,two PBRs were constructed,namely bacterial-microalgal symbiosis system( unsterilized ADE influent) and individual microalgae system( sterilized ADE influent), and the treatment efficiency of ADE and biomass accumulation of microalgae were compared. The results showed that the bacterial-microalgal symbiosis system significantly improved the treatment efficiency of ADE compared with the individual microalgae system,and the removal rates of TN,TP and COD reached up to( 82. 1 ± 1. 9) %,100% and( 83. 5 ±1. 8) %,respectively. Three-dimensional fluorescence spectrum analysis showed that the composition and peak value of fluorescent organic matters in ADE changed after sterilization. Compared with the individual microalgae system, the bacterial-microalgal symbiosis system showed significant advantages in the degradation of fluorescent organic matters,in which the macromolecular protein organic matters were effectively degraded into small molecule humic acid organic matters. Therefore,the results demonstrated that the bacterial-microalgal symbiosis system could be applied in microalgae expansion cultivation and simultaneous treatment of ADE. The system could save the cost of high temperature autoclave sterilization and obtain more ideal ADE treatment effect.更多还原