【摘要】 目的探讨山姜素对角叉菜胶致小鼠急性炎症的抗炎作用及机制。方法按照体重将昆明小鼠随机分为5组:正常组、模型组和低、中、高3个剂量实验组,每组10只。正常组和模型组灌胃0.9%Na Cl,3个剂量实验组分别灌胃山姜素10,20,40 mg·kg^-1,每天1次,连续给药7 d。末次给药1 h后,用角叉菜胶复制足肿胀小鼠模型。以酶联免疫吸附法检测肿胀足组织中髓过氧化物酶（MPO）活性、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平,以免疫印迹法检测肿胀足组织中过氧化物酶增殖物活化受体γ（PPARγ）的表达和核因子-кB P65（NF-κB p65）磷酸化水平。结果正常组、模型组和低、中、高3个剂量实验组的MPO分别为（1.15±0.05）,（1.90±0.35）,（1.71±0.26）,（1.54±0.28）和（1.22±0.31）U·g^-1;上述这5组的TNF-α分别为（176.3±43.6）,（324.9±45.8）,（299.3±27.7）,（273.8±27.8）和（256.3±26.1）pg·g^-1;上述这5组的IL-1β分别为（299.3±10.7）,（472.1±39.8）,（414.5±26.5）,（387.6±10.3）和（346.8±28.0）pg·g^-1;上述这5组的PPARγ蛋白表达量（灰度值）分别为0.93±0.05,0.48±0.05,0.58±0.05,0.67±0.06和0.84±0.06;上述这5组的NF-κB p65磷酸化水平（灰度值）分别为0.22±0.04,1.08±0.09,0.83±0.07,0.67±0.05,0.39±0.03。上述指标:模型组与正常组相比,差异均有统计学意义（均P<0.01）;3个剂量实验组与模型组相比,差异均有统计学意义（P<0.05,P<0.01）。结论山姜素对角叉菜胶诱导的急性炎症有抗炎作用,其机制可能与PPARγ/NF-κB信号通路有关。更多还原

【Abstract】 Objective To explore the anti-inflammatory effects and mechanism of alpinetin on acute inflammation caused by carrageenan in mice.Methods Kunming mice were randomly divided into five groups:normal group（0.9%Na Cl）,model group（0.9%Na Cl）and low,middle,high dose experimental groups(alpinetin:10,20,40 mg·kg^-1),each group had ten mice,once per day for seven days.One hour after the last administration,paw edema model was established using carrageenan.The levels of the myeloperoxidase（MPO）activity,tumour necrosis factor-α（TNF-α）and interleukin-1β（IL-1β）in paw edema tissue were detected by enzyme-linked immune sorbent assay.The expression of peroxisome proliferator-activated receptorγ（PPARγ）and nuclear factor kappa-B p65（NF-κB p65）in inflammatory paw tissue wereassayed by Western blotting.Results The MPO activities in normal group,model group and low,middle,high dose experimental groups were（1.15±0.05）,（1.90±0.35）,（1.71±0.26）,（1.54±0.28）,and（1.22±0.31）U·g^-1,respectively;TNF-αlevels in the above 5 groups were（176.3±43.6）,（324.9±45.8）,（299.3±27.7）,（273.8±27.8）,and（256.3±26.1）pg·g^-1,respectively;IL-1βlevels in the above 5 groups were（299.3±10.7）,（472.1±39.8）,（414.5±26.5）,（387.6±10.3）,and（346.8±28.0）pg·g^-1,respectively;the expression（grey value）of PPARγin the above 5 groups were 0.93±0.05,0.48±0.05,0.58±0.05,0.67±0.06,and 0.84±0.06,respectively;phosphorylation level（grey value）of NF-κB p65 in the above 5 groups were0.22±0.04,1.08±0.09,0.83±0.07,0.67±0.05,and 0.39±0.03.Comparing between model group and normal group,the differences of the factors were significant（all P<0.01）;comparing between the three dose of experimental groups and model group,the differences of the factors were significant（P<0.05,P<0.01）.Conclusion Alpinetin displayed significant anti-inflammatory effects on acute inflammation induced by carrageenan,which might be related to the regulation of PPARγ/NF-κB signaling pathway.更多还原