【摘要】 目的探讨重组人肿瘤坏死因子-α（rhTNF-α）联合环磷酰胺（CTX）对胶质瘤大鼠的增殖抑制作用及机制。方法通过颅脑注射大鼠胶质瘤C6细胞构建胶质瘤大鼠模型。取60只制备成功的胶质瘤大鼠模型,随机分为4组,每组15只。实验组-1和实验组-2经颈总动脉分别予以注射rhTNF-α10μg·kg^-1,CTX 15μg·kg^-1,实验组-3先予以经颈总动脉注射rhTNF10μg·kg^-1,120 min后,再予以注射CTX 15μg·kg^-1,模型组注射等量0.9%NaCl。观察各组大鼠存活周期及肿瘤体积,并检测实验组-2和实验组-3大鼠胶质瘤组织中CTX含量。结果模型组,实验组-1,实验组-2,实验组-3大鼠的存活时间分别为（12.69±2.54）,（16.92±2.37）,（25.23±2.26）,（34.59±3.05） d,肿瘤体积分别为（442.63±16.59）,（339.56±42.15）,（156.28±23.15）,（115.26±20.15） mm³。模型组分别与实验组-1,实验组-2,实验组-3比较,差异均有统计学意义（均P <0.05）;实验组-3分别与实验组-1和实验组-2比较,差异均有统计学意义（均P <0.05）。实验组-2和实验组-3大鼠的胶质瘤组织中CTX含量分别为（1.96±0.52）,（8.63±2.54）μg·mL^-1,差异有统计学意义（P <0.05）。结论 rhTNF-α联合CTX可协同抑制胶质瘤生长,延长大鼠生存期,与rhTNF-α可调节血脑屏障增加瘤组织中CTX含量有关。更多还原

【Abstract】 Objective To explore the proliferation inhibitory effect of recombinant human tumor necrosis factor-α（rhTNF-α） in combination with cyclophosphamide（CTX） in glioma rats.Methods The glioma rat model was established by intracranial injection of rat glioma C6 cells.Sixty glioma rats successful modeled were randomly divided into 4 groups,with 15 rats in each group.The rats in experimental group-1 and experimental group-2 given common carotid artery injection with rhTNF-α(10 μg·kg^-1) and CTX(15 μg·kg^-1),respectively.The rats in experiment group-3 were injected with CTX(15 μg·kg^-1) 120 min after the rhTNF-α(10 μg·kg^-1) injection,while the model group was injected with the same amount of 0.9% NaCl.The survival time and tumor volume were observed in each group,and the content of CTX in experimental group-2/-3 were detected.Results After rhTNF-αwas injected into glioma rats,the opening of blood tumor barrier gradually increased and then decreased,and the content of Evans blue（EB） reached the peak at 120 min after injection.The survival time of model group, experimental-1/-2/-3 group were（12.69 ± 2.54）,（16.92 ± 2.37）,（25.23 ± 2.26）,（34.59 ± 3.05） d,respectively; the tumor volume were（442.63 ± 16.59）,（339.56 ± 42.15）,（156.28 ± 23.15）,（115.26 ± 20.15） mm3,respectively.There were significant differences between the model group and the experimental group-1/-2/-3（P < 0.05）.Additionally,there were significant differences between the experimental group-3 and the experimental group-1/-2（P < 0.05）.The content of CTX in glioma tissue of experimental group-2/-3 were（1.96 ± 0.52）,（8.63 ± 2.54） μg·mL^-1,respectively,and significant difference was found between the two groups（P < 0.05）.Conclusion RhTNF-α combined with CTX can inhibit the growth of glioma and prolong the survival time of rats,which is related to RhTNF-α which can regulate the blood brain barrier and increase the content of CTX in glioma tumor tissue.更多还原