【摘要】 本研究通过运用Biolog技术,对新疆不同类型盐碱地土壤微生物群落特征进行研究,探讨不同盐碱土壤对微生物碳源代谢和微生物群落的影响。结果表明:不同类型盐碱土壤微生物碳源利用能力存在差异,表现为:碳酸盐型>硝酸盐型>氯化-硫酸盐型>硫酸盐型。主成分分析(PCA)表明,碳水化合物类和羧酸类是造成碳源利用分异的主要碳源。微生物Shannon指数(H)多样性由大到小为:碳酸盐群落>氯化-硫酸盐群落>硝酸盐群落>硫酸盐群落,在Simpson指数(D)和McIntosh指数(U)上硫酸盐型群落均显著(P﹤0.05)低于其他三类群落。相关分析表明有机质与多样性指数Shannon指数(H)、Simpson指数(D)有极显著相关性(P﹤0.01),与全磷有显著(P﹤0.05)相关。冗余分析(RDA)表明,pH、全盐、速效钾对微生物代谢有极显著(P﹤0.01)影响,全磷有显著(P﹤0.05)影响,而速效磷的影响十分显著(P﹤0.001)。总体来说,碳酸盐型土壤微生物群落有较强的碳源利用能力和较高功能多样性,而土壤速效磷、有机质能够促进盐碱地微生物碳源代谢活性和群落多样性。更多还原

【Abstract】 The objective of this study was to evaluate the effect of different types of saline-alkali soil on soil microbial carbon metabolism and microbial community in Xinjiang, northwest China. BIolog method was performed to investigate characteristics of soil microbial in different soil types. The results indicated that soil types significantly affect the carbon utilization capacity, which decreased in an order carbonate communities>nitrate communities>chloride-sulfate communities>sulfate communities. The principal component analysis(PCA) showed that carbohydrates and carboxylic acids were the main carbon sources that cause the differentiation of carbon sources utilization. Shannon index(H) in the whole soil decrease in the order of carbonate community> chloride-sulfate community> nitrate community> sulfate community. We also observed that sulfate communities were significantly lower than the other types of communities on Simpson index(D) and McIntosh index(U)(P<0.05). Correlation analysis indicated that soil organic matter(SOM) significantly related with diversity index(Shannon, Simpson and McIntosh) and total P. Redundancy analysis(RDA) revealed that pH, total salt, available K, available P and total P were the main factors in influencing soil microbial carbon metabolism. Among them, soil available P effect was extremely significant(P<0.05). Together, carbonate community has strong carbon source utilization ability and high microbial diversity, available P and SOM can promote carbon sources metabolism and microbial community structure diversity in study area.更多还原