【摘要】 目的探讨组蛋白乙酰化修饰位点H2BK16a在慢性哮喘模型小鼠肺组织中的表达变化。方法 BALB/C小鼠分为正常组和慢性哮喘组,应用卵蛋白致敏和激发方法构建慢性哮喘模型,HE染色观察气道炎症浸润,AB-PAS染色观察气道杯状细胞增生,Masson染色观察上皮下胶原沉积,免疫组织化学染色观察H2BK16ac在肺组织中的表达分布,Western blot检测肺组织中H2BK16ac水平。结果慢性哮喘小鼠气道血管周围可见大量炎症细胞浸润、气道纤毛柱状上皮细胞中的杯状细胞增生、黏液腺化生、上皮下胶原沉积,表明构建慢性哮喘模型成功。免疫组织化学染色和Western blot检测显示,正常小鼠肺组织中H2BK16ac的表达极少;慢性哮喘小鼠肺组织中H2BK16ac的表达水平显著升高,在气道纤毛柱状上皮细胞、气道血管周围炎症细胞、血管平滑肌细胞、肺泡腔炎症细胞中H2BK16ac均呈明显阳性表达。结论肺组织中乙酰化修饰位点H2BK16ac的高表达可能与慢性哮喘的发生密切相关。更多还原

【Abstract】 Objective To investigate the expression change of histone acetylation site H2BK16ac in the lung tissue during asthma pathogenesis. Methods BALB/C mice were divided into control group and asthma group. Chronic asthma model was established by ovalbumin sensitization and stimulation. Then airway inflammatory cells infiltration was observed by HE staining, proliferation of goblet cells was detected by AB-PAS staining, and the subepithelial collagen deposition was detected by masson staining. The expression distribution of histone acetylation site(H2BK16ac) in the lung tissue was detected by immunohistochemistry. The expression level of H2BK16ac was quantified by Western blot. Results A large number of inflammatory cells infiltration around the airway vessels, goblet cell proliferation among airway ciliated columnar epithelial cells, mucus gland metaplasia, and subepithelial collagen deposition were observed in the asthma group, indicating the successful establishment of chronic asthma model. Immunohistochemical staining and Western blot showed that the expression level of H2BK16ac was extremely low in the lung tissue of normal group. While the expression of H2BK16ac in the lung tissue of chronic asthmatic mice was significantly increased, and the expression of H2BK16ac in airway ciliated columnar epithelial cells, perivascular inflammatory cells, vascular smooth muscle cells and alveolar inflammatory cells in asthmatic group was strongly positive. Conclusion Histone acetylation modification site H2BK16ac in lung tissue may be closely related to asthma pathogenesis.更多还原