【摘要】 以1-乙基-3-(3-二甲基氨基丙基)碳二亚胺盐酸盐(EDC·HCl)为羧基活化剂,己二酸二酰肼(ADH)为交联剂,制备了生物活性聚(L-谷氨酸)(PLGA)水凝胶.通过X射线衍射和扫描电子显微镜等表征了在不同浓度模拟体液(SBF)中羟基磷灰石(HA)的形成和生长. PLGA水凝胶的表面和内部均可观察到HA的形成和生长.同时探讨了PLGA水凝胶矿化前后的力学性能.将矿化前后PLGA水凝胶用于脂肪干细胞(ASCs)的培养,研究其细胞相容性.更多还原

【Abstract】 Bioactive poly(L-glutamic acid)( PLGA) hydrogels were prepared by using adipic acid dihydrazide( ADH) as a crosslinking agent. The formation and growth of hydroxyapatite( HA) in the simulated body fluid( SBF) of different concentrations were characterized by various techniques. PLGA is a kind of anionic poly( amino acid) with abundant carboxylic groups,which favors the enrichment of calcium ions and formation of HA. The growth of HA with soaking time was detected at the surface and interior of the PLGA hydrogels.The mechanical properties of the PLGA hydrogels before and after mineralization were studied in dynamic rheology experiments. Being cytocompatible,the mineralized PLGA hydrogels were successfully applied for cultivation of adipose-derived stem cells( ASCs). These results presented a method of preparing HA/PLGA composite hydrogels,which might be used as a promising biomaterial for bone tissue engineering.更多还原