【摘要】 发光细菌能够发出波长为450～490 nm的可见光,其发光强度随待测溶液中毒性物质浓度增加而减弱。发光细菌法具有简单、快速和高稳定性等特点,被广泛应用于水质急性毒性分析。近年来,随着微流控技术微型化、集成化和自动化的不断发展,基于微流控技术的发光细菌毒性分析装置的研究越来越多。与传统方法相比,微流控型生物传感器所需菌液少,可以有效地避免人为误差和外界条件干扰等,灵敏度更高。本文结合发光细菌的特点和发光机制,对微流控型生物发光传感器及其在环境监测中的应用进展进行了评述。更多还原

【Abstract】 Luminescent bacteria are a kind of bacteria which emit visible light at 450 nm-490 nm. Its luminous intensity decreases with the increase of the concentration of toxic substances in test solution. The methods hased on luminescent bacteria are widely used in acute toxicity analysis of water quality because of its sensitiveness,quickness and inexpensiveness. With the rapid development of genetic modification technology,non-luminescent bacteria can also produce visible light by planting luminescent genes,which makes the application field of luminescent bacteria widen. In recent years,biosensors based on luminescent bacteria have attracted more and more attention,and the reports of bioluminescence biosensors based on microfluidic systems are increasing day by day. Based on the characteristics and mechanism of luminescent bacteria,this review introduced their applications in the environmental monitoring,and summarized several new bioluminescence sensors.更多还原