【摘要】 【目的】内生真菌印度梨形孢Piriformospora indica(Pi)可定殖在多种植物根系中,促进植物生长发育,增强植物抗逆性。【方法】将印度梨形孢接种至8种供试培养基中,测定其在8种培养基中的生长速率,并将印度梨形孢与水稻幼苗共培养,分析印度梨形孢对水稻幼苗株高、叶长、根长、根数、鲜重和叶绿素含量等生物学性状的影响。【结果】8种供试培养基中,V8培养基是最适合印度梨形孢生长的培养基,且加入一定量蔗糖能进一步促进印度梨形孢的生长;研究还发现,接种了印度梨形孢的水稻株高、叶长、根长、根数、叶绿素含量和地上地下部鲜质量都显著高于对照;与对照相比,接种印度梨形孢20 d后的水稻,其株高增高了32.36%,叶绿素含量提高了15.88%。【结论】印度梨形孢是通过增加光合作用和增强根系生理机能,从而促进水稻的生长发育。更多还原

【Abstract】 【Objective】 Enhancement on the growth, development and stress resistance of rice plants from the colonization of the endophytic fungus Piriformospora indica(Pi) on the roots was investigated. 【Method】 Pi was inoculated on 8 different media for optimal culture and co-cultured on rice seedlings to determine the biological improvements on the plants. 【Result】Medium V8 was found to be most desirable for Pi growth. Addition of a certain amount of sucrose would further increase the effect. The plant height, leaf length, root length, root count, chlorophyll content and quality of the above-and underground parts of the treated rice plants were significantly greater than those of control. Notably, 20 days after the inoculation, the plant height increased by 32.36% and chlorophyll content 15.88%. 【Conclusion】 Pi significantly promoted the growth and development of rice plants as it symbiotically enhanced the photosynthesis and root physiology of the host.更多还原