【摘要】 为研究武汉城市湖泊抗生素及抗生素抗性基因的污染水平与分布特征,文章采用超高效液相色谱-质谱法和荧光定量PCR法对南湖、沙湖和东湖水体及底泥中12种抗生素、9种ARGs及Ⅰ类整合子intI1进行定性和定量分析。结果表明,湖泊水体及底泥中均以喹诺酮类抗生素污染为主,浓度范围分别为51.43～105.62 ng/L和9.01～12.93 ng/g。10种目标基因中,tetG、tetM、sul1、sul2、qnrD及intI1的检出率均为100%。磺胺类抗性基因sul1的相对丰度最高(2.39×10^-2～3.99×10^-1),属于优势抗性基因。intI1含量与4种抗性基因含量（tetG、tetM、sul1及qnrD）、ARGs总量之间均存在显著正相关关系（P<0.05）,说明intI1是ARGs在城市湖泊环境中进行水平基因转移的重要媒介。冗余分析表明四环素类、喹诺酮类抗生素污染和intI1含量是影响湖泊水体、底泥中ARGs丰度及分布的重要因素。更多还原

【Abstract】 In order to investigate the pollution status and distribution characteristics of antibiotics and antibiotic resistance genes（ARGs） in urban lakes of Wuhan, high performance liquid chromatography-mass spectrometry and quantitative PCR were applied to analyze the occurrence and quantities of twelve antibiotics, nine ARGs and class I integron gene intI1 in water and sediment samples of South Lake, Sand Lake and East Lake. The results showed that quinolones were the main contaminants in all samples. The total concentration of quinolones ranged from 51.43 to 105.62 ng/L in water and from 9.01 to 12.93 ng/g in sediments, respectively. Among the ten tested genes, tetG, tetM, sul1, sul2, qnrD as well as intI1 had 100% detection frequency. Sul1 had the highest relative abundance(2.39×10^-2～3.99×10^-1), and was the predominant resistance gene. There was a significant positive correlation between the relative abundance of intI1 and four resistance genes（tetG, tetM, sul1 and qnrD） as well as total ARGs（P<0.05）, indicating that intI1 was a significant media for horizontal gene transfer of ARGs in urban lakes. And redundancy analysis showed that tetracyclines, quinolones and intI1 may play a very important role in the abundance and distribution of ARGs in urban lakes of Wuhan.更多还原