【摘要】 目的:探讨呼吸道合胞病毒(RSV)、季节性流感病毒A型(Inf-A)混合感染患儿体内常见的炎症因子表达水平及其临床意义。方法:选取Inf-A和RSV检测阳性的患儿及健康儿童共95例纳入研究,将所有研究对象分为四组:Inf-A感染组、RSV感染组、混合感染组(MIX组)及健康对照组(HC组)。采用悬液芯片技术和酶联免疫吸附试验(ELISA)法分别检测四组患儿体内IL-1β、IL-2、IL-8、TNF-α、IFN-γ等致炎因子及IL-4、TGF-β等抗炎细胞因子的表达水平,比较分析四组患儿的炎症因子水平与病情严重程度的相关性。结果:急性期,Inf-A感染组、RSV感染组、MIX组患儿IL-1β、IL-8、TNF-α、IFN-γ水平均不同程度升高,MIX组IL-1β、TNF-α、IL-8水平升高更明显。在疾病恢复期,IL-1β、TNF-α、IL-8、IFN-γ水平较治疗前均明显下降,但与HC组相比TNF-α、IFN-γ水平仍明显偏高; RSV组、Inf-A组及MIX组IL-4与TGF-β较治疗前明显下降,与HC组比较差异无统计学意义(P>0. 05)。结论:Inf-A、RSV混合感染患儿体内致炎性因子占主导地位,以IL-1β、IL-8、TNF-α水平升高为主,而IL-8水平的变化可能是反映病情严重程度较敏感的指标。更多还原

【Abstract】 Objective: To probe into the expression levels and clinical significance of serum inflammatory factors in children with respiratory syncytial virus( RSV) and influenza A virus( Inf-A). Methods: A total of 95 children with positive Inf-A and RSV and some healthy children were enrolled in the study. All subjects were divided into 4 groups: Inf-A infection group,RSV infection group,mixed infection group( MIX group) and healthy control( HC group). Enzyme-linked immunosorbent assay( ELISA) was used to detect the expression levels of inflammatory factors such as IL-1β,IL-2,IL-8,TNF-α,IFN-γ and anti-inflammatory cytokines such as IL-4 and TGF-β in four groups. The correlation between inflammatory factor levels and severity of disease was compared and analyzed among four groups. Results: The levels of IL-1β,IL-8,TNF-α and IFN-γ in the Inf-A infection group,the RSV infection group and the MIX group increased to different degrees in the acute phase,and the levels of IL-1β,TNF-α and IL-8 in the MIX group increased more significantly. In the recovery period,the levels of IL-1β,TNF-α,IL-8 and IFN-γ were significantly lower than those before treatment,yet the levels of TNF-α and IFN-γ were still significantly higher than those in the HC group. Expression of IL-4 and TGF-β decreased significantly in the RSV group,the Inf-A group and the MIX group compared with before treatment,and there was no statistical significance compared with the HC group. Conclusion: The inflammatory factors increased predominantly in children infected with Inf-A and RSV,mainly concentrated in the levels of IL-1β,IL-8 and TNF-α,while the changes in the levels of IL-8 may be the sensitive indicators of the severity of the response.更多还原