【摘要】 本文利用光学显微镜、电子显微镜技术研究了30 nm粒径圆球形纳米银颗粒及30 nm粒径圆球形纳米氧化锌颗粒对双眉虫(Diophrys sp.)细胞超微结构的影响。结果显示,这两种纳米材料均对细胞结构造成了一些相似的影响,主要表现在:(1)细胞内形成大量空泡;(2)线粒体出现肿胀、内部嵴断裂以及局部结构模糊和变空的现象;(3)细胞内出现大量自噬体,以及自噬后形成的髓鞘样小体。此外,纳米银颗粒处理组还出现细胞核核仁消失,核膜界限模糊的现象;纳米氧化锌处理组还发生了纤毛杆处质膜融合,细胞中产生大量溶酶体的现象。初步推测,纳米银颗粒主要通过影响细胞核功能,进而造成其他生命活动紊乱,而纳米氧化锌颗粒则会诱导细胞凋亡和破坏纤毛器结构从而限制细胞运动。更多还原

【Abstract】 The effects of 30 nm sized spherical nano silver-particles( Ag-NPs) and 30 nm sized spherical nano-zinc oxide particles( Zn O-NPs) on the ultrastructure of Diophrys sp. cells were studied by optical microscopy and electron microscopy. The results showed that both of the nanomaterials have similar influences on the ultrastructure of cells which included:( 1) cytoplasmic vacuolation;( 2)mitochondria swelling,with their internal cristae rupturing and disappeared; and( 3) a large number of autophagosomes and myelinlike bodies forming after autophagy appear in the cells. In addition,the Ag-NPs treatment also caused the nucleolus disappearance,and the nuclear membrane blurred,and the Zn O-NPs caused the ciliary membrane fusion and induced the cells to produce a large number of lysosomes. According to available data,it is speculated that Ag-NPs mainly affect life activities by impeding nuclear function,while Zn O-NPs destroy the structure of the ciliary organelle,thus induce the apoptosis and restrict cell movement.更多还原