【摘要】 目的:基于体外实验和分子对接研究枫杨抑菌活性及机制。方法:以枫杨果、树皮、叶为研究对象,分别研究其水提物和醇提物(70%乙醇)对金黄色葡萄球菌、大肠杆菌、绿脓杆菌、伤寒沙门菌、化脓性链球菌、表皮葡萄球菌的抑菌活性,确定抑菌谱和最小抑菌浓度(MIC)。利用活性追踪方法,通过硅胶吸附色谱、薄层色谱等手段,分离单体化合物,并进行抑菌活性研究,选择PBP5、FabI、FabH、Fts Z 4个抑菌关键蛋白靶点,利用Systems Dock Web Site软件进行分子对接,研究其抑菌机制。结果:实验发现枫杨果的醇提物抑菌效果最好,对伤寒沙门菌最小抑菌浓度为7.81 mg/mL,首次从枫杨果中分离得到β-谷甾醇、β-胡萝卜苷、齐敦果酸,三个化合物对于PBP5、FabH、FtsZ蛋白靶点Docking score均大于7,结果活性很好。结论:枫杨果醇提物抑菌谱广、抑菌能力强,其可能是通过抑制革兰氏阳性菌细胞壁肽聚糖的合成,以及细菌脂肪酸的生物合成,干扰其生物膜的形成从而抑制细菌的生长和繁殖,从而起到广谱的抗菌效果。更多还原

【Abstract】 Objective: Based on in vitro experiments and molecular docking, the antibacterial activity and mechanism of Pterocarya stenoptera C. DC were studied. Methods: The antibacterial activity of water extract and alcohol extract(70% ethanol) of pterocarya stenoptera C. DC, bark and leaf against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi,Streptococcus pyogenes and Staphylococcus epidermidis were studied, and the antibacterial spectrum and Mic were determined. Using the activity tracking method, the single compound was isolated by means of silicone adsorption spectrum and thin-layer chromatography,and the study of anti-bacterial activity was carried out, and four key protein targets of antiseptic protein were selected, and molecular docking was carried out by using Systems Dock Web Site software. The mechanism of antiseptic stoids was studied. Result: The results showed that the ethanol extract of pterocarya stenoptera C. DC had the best bacteriostatic effect. The minimum bacteriostatic concentration was 7.81 mg/ml for Salmonella typhi. β-sitosterol, β-carotene and oleanolic acid were isolated from pterocarya stenoptera C. DC for the first time. The three compounds were more than 7 for the docking score of PBP5, FabH and FtsZ protein targets,and the activity was very good. Conclusion: The alcohol extract of Fengyang fruit has a wide antibacterial spectrum and strong antibacterial ability. It may interfere with the formation of biofilm by inhibiting the synthesis of peptidoglycan in cell wall of Gram-positive bacteria and the biosynthesis of bacterial fatty acid, so as to inhibit the growth and reproduction of bacteria, thus playing a broad-spectrum antibacterial effect.更多还原