【摘要】 为了快速检测猪德尔塔冠状病毒(PDCoV),针对PDCoV N基因设计特异的引物,建立了猪德尔塔冠状病毒重组聚合酶扩增(RPA)方法。特异性试验中以PPRSV、PEDV、PKV、TGEV和FMDV为模板时均未产生任何条带,特异性较好。该检测方法的灵敏度为103 copies/μL,敏感性较好。用该方法检测194份仔猪腹泻样品,PDCoV阳性率为14.4%,较常规RT-PCR阳性率为11.9%更高。研究所建立的猪德尔塔冠状病毒RPA检测方法具有反应快速、操作简单、结果可靠、设备要求低等优点,可为基层简易实验室提供一种方便适用的快速检测方法。更多还原

【Abstract】 In order to detect porcine deltacoronavirus(PDCoV)rapidly,specific primers were designed and synthesized based on the conserved sequence of PDCoV N gene.A porcine deltacoronavirus recombinase polymerase-mediated amplification(RPA)method was established,and the sensitivity and specificity of the method were evaluated.The results showed that there was no amplification when using porcine reproductive and respiratory syndrome virus,porcine epidemic diarrhea virus,porcine Krubu virus,porcine transmissible gastroenteritis virus and foot-and-mouth disease virus as templates,indicating that this method has good specificity;The sensitivity of this detection method is 103 copies/μL,indicating that this method has good sensitivity.Using this method to detect 194 samples of piglet diarrhea,the positive rate of PDCoV was14.4%,which was better than the results of routine RT-PCR(11.9%).It indicated that this method can be used for clinical detection of PDCoV.The RPA detection method established by this study is rapid,easy to operate,reliable and low in equipment requirements.It provided a convenient and applicable rapid detection method for simple laboratories.更多还原