【摘要】 牛病毒性腹泻(BVD)是由牛病毒性腹泻病毒(BVDV)所致牛的一种接触性传染病,呈世界性流行,该病主要表现为腹泻、急慢性黏膜感染、繁殖障碍等,对畜牧业危害严重,是国际贸易中动物检疫的重要疫病之一。快速准确的检测手段,有利于该传染病的早期发现和及时控制。目前,用于检测牛病毒性腹泻病毒的方法很多,论文对近年来国内外应用的等温核酸扩增技术(IAT)、重组酶聚合酶扩增技术(RPA)、指数富集配体系统进化技术(SELEX)、多重PCR技术、多重连接探针扩增技术(MLPA)、RT-PCR抗原酶联免疫吸附试验(ELISA)和间接免疫荧光试验(IFA)等进行简要介绍,为我国动物检疫工作提供参考。更多还原

【Abstract】 Bovine viral diarrhea is caused by bovine viral diarrhea virus,which manifests many symptoms,such as diarrhea,acute and chronic mucosal infection and reproductive disturbance.It is seriously harms to animal husbandry and also one of the important animal diseases of animal quarantine in international trade.Rapid and accurate detection is conducive to early detection and control of this disease.At present,there are many methods for detecting bovine viral diarrhea virus.In this paper,we introduced the detection technologies including IAT,RPA,SELEX,multiplex PCR,MLPA,RT-PCR ELISA and IFA,in order to provide reference for rapid and highly effective detecting techniques in the work of animal inspection and quarantine.更多还原