【摘要】 目的:结合体外活性实验,优选美洲大蠊抗肺纤维化活性部位（ML-HB）的最佳提取工艺。方法:采用单因素实验和L₉（3⁴）正交实验设计考察提取温度、溶剂浓度、提取时间、提取次数等因素对美洲大蠊抗肺纤维化活性部位（ML-HB）提取工艺的影响,并结合MTT取工艺。结果:料液比为1:5时（g/mL）最佳提取工艺为:提取温度为70℃,以70%的乙醇提取3次,提取时间为2.5 h/次。对此工艺进行验证,活性部位得率均值为0.59%,其24 h72.40、71.03和72.26μg/mL,72 h的IC₅₀值分别为67.24、69.76和67.22μg/mL。结论:建立了ML-HB的最佳提取工艺,为美洲大蠊抗肺纤维化的研发提供了依据。更多还原

【Abstract】 Objective: Combined with the activity test in vitro, the extraction technology of the anti-pulmonary fibrosis active site（ML-HB）from Periplaneta americana was optimized. Methods: The single factor experiment and L₉（3⁴）orthogonal experiment were used to optimize extraction temperature, solvent concentration, extraction time and the number of extraction times of anti-pulmonary fibrosis active site of Periplaneta americana, and combined with MTT method, the anti-pulmonary fibrosis activity fraction from the different extracts was detected, the yield and IC₅₀ value of the active site were used as evaluation indicators to determine the optimal extraction process of the active site of Periplaneta americana. Results: When the ratio of material to liquid was 1:5（g/mL）, the optimal extraction process of the anti-pulmonary fibrosis active site of Periplaneta americana was determined as follows: the extraction temperature was 70 ℃, the ethanol concentration was 70%, the number of extraction times was 3, and the extraction time was 2.5 h.Verifying the optimum conditions, the ML-HB average extraction yield was 0.59%. The IC₅₀ values at 24 h were 77.77, 75.17 and76.03 μg/mL, respectively; 72.40, 71.03 and 72.26 μg/mL at 48 h, respectively; 67.24, 69.76 and 67.22 μg/mL at 72 h, respectively.Conclusion: It stablishes the best extraction technology of ML-HB, and it provides the basis for the research and development of antipulmonary fibrosis of Periplaneta americana.更多还原