【摘要】 目的:探讨高血氨对小鼠肝细胞损伤及空间学习记忆能力的影响。方法:将实验小鼠随机分为实验组、对照组。实验组小鼠腹腔注射3. 75%氯化铵溶液,对照组腹腔注射等量的生理盐水; Morris水迷宫观察小鼠空间学习记忆能力的变化;摘眼球、断尾取血测血氨含量;取肝脏组织制作石蜡切片,显微镜观察肝脏的组织形态结构。结果:对照组一般状态均较好;实验组表现为行动缓慢,精神不振,皮毛缺乏光泽,体质量与给药前比较差异无统计学意义(P>0. 05),且与对照组比较差异无统计学意义(P>0. 05)。血氨测定:与对照组比较,实验组小鼠的血氨明显升高,差异有统计学意义(P<0. 05)。Morris水迷宫实验:各组内小鼠1～5天5个时间点逃避潜伏期有显著性差异;与对照组比较,实验组第1～5天5个时间点逃避潜伏期明显延长,跨平台次数明显减少,差异有统计学意义(P<0. 05);显微镜观察肝脏组织结构:实验组出现小鼠肝组织气球样变、脂肪变性以及坏死。结论:高血氨可导致小鼠肝脏组织变性、坏死以及空间学习记忆能力下降。更多还原

【Abstract】 Objective: To investigate the effects of hyperammonia on liver tissue and spatial learning and memory in mice.Methods: The experimental mice were randomly divided into experimental group and control group. The mice in the experimental group were injected with 3. 75% ammonium chloride solution and the control group were injected with the same amount of normal saline. Morris water maze observed the changes of spatial learning and memory in mice. Blood samples were taken from eyeball and tail to measure blood ammonia content. The liver tissue was prepared into paraffin sections and the histological structure of the liver was observed by microscope. Results: The general condition of the control group was good. The experimental group showed slow movement,low spirit,lack of luster of fur,and no difference in body weight( P>0. 05) before administration,and no difference from the control group( P>0. 05). Determination of serum ammonia: compared with the control group,serum ammonia in the model group was significantly increased,with statistically significant difference( P<0. 05). Morris water maze experiment: there were significant differences in the escape incubation period of mice in each group at 5 time points in 1 ～ 5 days. Compared with the control group,the escape latency of the experimental group at five time points of 1 ～ 5 days was significantly prolonged,and the number of cross-platform times was significantly decreased,with statistically significant differences( P< 0. 05). Microscopic observation of liver tissue structure showed balloon-like degeneration,steatosis and necrosis in the experimental group. Conclusion: Hyperammonia can cause degeneration and necrosis of mouse liver and decrease of spatial learning and memory.更多还原