【摘要】 目的研究敌百虫和毒死蜱联合染毒对小鼠脑组织的氧化损伤作用,为预防和及时治疗提供实验依据。方法敌百虫组:剂量22.5 mg/kg(1/20 LD50);毒死蜱组:剂量3.0 mg/kg(1/20 LD50);联合1组:敌百虫18.0 mg/kg(1/25 LD50)+毒死蜱2.4 mg/kg(1/25 LD50);联合2组:敌百虫22.5 mg/kg(1/20 LD50)+毒死蜱3.0 mg/kg(1/20 LD50);联合3组:敌百虫30.0 mg/kg(1/15 LD50)+毒死蜱4.0 mg/kg(1/15 LD50)。各组小鼠按照0.1 mL/kg灌胃染毒,每天1次,连续7 d,对照组给予等体积生理盐水。末次灌胃12 h后处死小鼠,检测脑组织中乙酰胆碱酯酶(AChE)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力,丙二醛(MDA)、脂质过氧化物(LPO)含量。结果与对照组比较,敌百虫组与毒死蜱组小鼠脑组织SOD、GSH-Px、CAT、LPO、MDA、AChE活力差异无统计学意义(P>0.05)。与对照组、敌百虫组或毒死蜱组比较,联合1、2、3组的小鼠脑组织T-SOD、GSH-Px、CAT、AChE活力降低(P<0.05),LPO含量升高(P<0.05),且呈量效关系。结论敌百虫和毒死蜱联合染毒可致小鼠脑组织氧化损伤,二者联合染毒具有一定的协同作用。更多还原

【Abstract】 Objective To study toxic effect of combined exposure to trichlorfon and chlorpyrifos on the oxidative damage of brain in mince, to provide experimental basis for prevention and timely treatment. Methods Trichlorfon group: given 22.5 mg/kg(1/20 LD50) trichlorfon;Chlorpyrifos group: given 3.0 mg/kg(1/20 LD50) chlorpyrifos;Combined group 1:given 18.0 mg/kg(1/25 LD50) trichlorfon and 2.4 mg/kg(1/25 LD50) chlorpyrifos;Combined group 2:given 22.5 mg/kg(1/20 LD50) trichlorfon and 3.0 mg/kg(1/20 LD50) chlorpyrifos;Combined group 3:given 30.0 mg/kg(1/15 LD50) and 4.0 mg/kg(1/15 LD50).Mice in each group were given 0.1 mL/kg gavage for poisoning once a day for 7 consecutive days.The control group was given normal saline of the same volume.Mice were sacrificed 12 hours after the last gavage,and activity of AChE,gsh-px,SOD,CAT,MDA and LPO in brain tissue were detected. Results Compared with the control group,the activity of SOD,gsh-px,CAT,LPO,MDA and AChE in the brain tissues of mice in trichlorfon group and chlorpyrifos group showed no significant difference(P>0.05).Compared with control group,chlorpyrifos group or chlorpyrifos group,the activity of t-sod,gsh-px,CAT and AChE in the brain tissues of mice combined with 1,2 and 3 groups decreased(P<0.05),and the content of LPO increased(P<0.05),showing a dose-effect relationship.Conclusion The combination of trichlorfon and chlorpyrifos could induce oxidative damage in brain tissue of mice,which has a certain synergistic effect.更多还原