【摘要】 在植物体内,FBPase酶是卡尔文循环内一个不可缺少的酶,在干旱、高温、盐碱等非生物胁迫中FBPase同样起着重要的作用。本试验从典型的耐极端低温的植物天山雪莲(Saussurea involucrata Kar.ER Kir.)中克隆了一段FBPase序列,命名为SikFBP1,同时也克隆出了一段SBPase序列命名为SikSBP。实时荧光定量显示2个基因对寒冷胁迫都做出了响应,为验证其抗寒性功能分别转入了烟草中。通过RT-PCR验证获得转化植株,低温胁迫下,通过丙二醛(MDA)含量、相对电子渗透率(REL)、过超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、PSⅡ最大光化学效率(Fv/Fm)及光合效率(Pn)的测量,发现转SikFBP1基因株系与转SikSBP基因株系在光合效率上提高较为微弱,但在耐寒性方面得到了一定的提高,为FBPase的功能研究提供一定参考。更多还原

【Abstract】 FBPase enzyme is an important enzyme in plant.Recent studies have shown that FBPase also plays an important role against abiotic stress such as high temperature and drought.In this study,aFBPase and a SBPase sequence were cloned fromSaussurea involucrata Kar.ER Kir.,a typical perennial alpine plant resistant to extreme low temperature,and was named as SikFBP1 and SikSBPrespectively.We found that both of them were responsive to low temperature by real-time fluorescence quantitative PCR.In order to verify the function of cold resistance of the sequence,SikFBP1 and SikSBPwere transferred into tobacco respectively.Transformed plants were obtained by RT-PCR verification.We also measured the content of malondialdehyde(MDA),relative electron transport(REL),superoxide dismutase(SOD)activity,catalase(CAT)activity,maximum photochemical efficiency(Fv/Fm)and photochemical efficiency(Pn).The results showed that compared with wild-type,the transgenic SikSBPplants and SikFBP1 plants were obviously improved in cold tolerance,but only slight increased in photosynthetic efficiency.更多还原