【摘要】 目的采用高效液相色谱法测定柴香疏肝胶囊中芍药苷的含量。方法采用高效液相色谱法,色谱柱为Inert Sus-tain C18柱(4. 6mm×150 mm,5μm),以乙腈-0. 1%磷酸溶液(14∶86)为流动相,流速为1. 0 m L/min,检测波长为230nm。结果芍药苷的线性范围为0. 1172～2. 344μg(r=0. 9999),芍药苷平均加样回收率为99. 05%,RSD为2. 08%。结论高效液相色谱法可以用于柴香疏肝胶囊中芍药苷的含量测定。更多还原

【Abstract】 Objective To determinate the content of Paeoniflorin in Chaixiangshugan Capsule by HPLC. Methods High perform-ance liquid chromatography( HPLC) method was used,which applied Inert Sustain-C18 column( 4. 6 mm × 150 mm,5 μm) and aceto-nitrile-0. 1% orthophosphoric( 14∶ 86) as mobile phase. The flow rate was 1. 0 m L/min,and detection wavelength was 230 nm. Re-sults The linear range was 0. 1172 ～ 2. 344 μg and correlation coefficient was 0. 9999. The average recovery was 99. 05%,and therelative standard deviation was 2. 08%. Conclusion HPLC method can be applied to the determination of Paeoniflorin in Chaixiang-shugan Capsule.更多还原