【摘要】 以肥城桃为材料,研究经NO、c-PTIO(NO消除剂)溶液及双蒸水处理(对照,CK)的桃果实在常温(25℃)和低温(0℃)贮藏下细胞膜脂过氧化相关酶活性和代谢产物的变化。结果表明:在低温贮藏期间,NO处理的桃果实丙二醛的含量仅为CK的29.4%,脯氨酸含量为CK的1.37倍,NO处理显著提高了抗氧化酶SOD、CAT、POD和APX的活性,分别为CK的1.31,1.48,1.32和1.65倍;在常温贮藏期间,c-PTIO处理的桃果实丙二醛含量为CK的2.52倍,脯氨酸含量为CK的58.8%,细胞膜通透性为CK的1.48倍,c-PTIO显著降低了桃果实SOD、CAT和POD的活性,仅为CK的88.1%,77.1%和72.1%。在冷信号胁迫下,NO处理的桃果实提高了相关抗氧化酶的活性,使渗透调节物质的含量增加,并降低了细胞膜的通透性和细胞膜脂过氧化程度,减少了对细胞膜的损害。更多还原

【Abstract】 To explore the effects of exogenous NO and cold stress on membrane lipid peroxidation-related enzymes activity and the changes of metabolites in ’Feicheng’ peach fruit after postharvest, peaches were treated with NO,c-PTIO solutions and double distilled water(CK) and then stored at room temperature(25 ℃) and low temperature(0 ℃). The results showed that during storage at low temperature, the content of MDA in peaches treated with NO was only 29.4% of the control, the content of proline was 1.37 times higher than that of the control. Besides, NO treatment increased the activities of SOD, CAT, POD and APX, which were 1.31, 1.48, 1.32 and 1.65 times higher than the control, respectively. During storage at room temperature, c-PTIO treatment increased the content of MDA,which was 2.52 times high than the control, and reduced the activities of SOD, CAT and POD, which were only88.1%, 77.1% and 72.1% of the control, respectively. At the same time, the content of proline decreased to 58.8%of the control, while the permeability of cell membrane was 1.48 times higher than the control. NO treatment combined with cold stress increased the activities of antioxidant enzymes in cells and the contents of osmotic regulation substances, reduced the permeability of cell membrane and membrane lipid peroxidation level, further reduced the damage to the cell membrane.更多还原