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自制间接法猪瘟抗体ELISA检测试剂盒性能研究

Performance of Swine Fever Antibody ELISA Test Kit by Self-developed Indirect Method

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【作者】 田锦李志敏王茂臻

【Author】 TIAN Jin;LI Zhi-min;WANG Mao-zhen;Beijing Vocational College of Agriculture;Beijing University of Agriculture;Beijing Yuying School;

【机构】 北京农业职业学院北京农学院北京市育英学校

【摘要】 为了评价实验室自行研发的间接法猪瘟抗体ELISA检测试剂盒的性能,我们用调试后的ELISA试剂盒和两个进口试剂盒(法国LSI公司及美国IDEXX公司产品),分别对184份临床样品进行了检测。结果显示:实验室初步建立的检测体系与LSI公司试剂盒符合率为83.6%、特异性100%、敏感性80.6%,与IDEXX公司试剂盒符合率为76.7%、特异性62%、敏感性93%。

【Abstract】 In order to evaluate the performance of swine fever antibody ELISA test kit by the indirect method developed by our laboratory, one ELISA kit and two imported kits(products of France LSI Company and American IDEXX Company) were used to test 184 clinical samples. The results show that the compliance rate of our initial established test system is up to 83.6% with the kit from LSI Company, the specificity is 100%, and the sensitivity is 80.6%; the compliance rate with the kit from IDEXX Company is 76.7%, the specificity is 62%, and the sensitivity is 93%.

【关键词】 猪瘟ELISA检测自制试剂盒
【Key words】 swine feverELISA testself-made kit
【基金】 北京农业职业学院科技研发推广类项目(XY-YF-16-30);北京市农委对接服务新型生产经营主体科技能力提升项目(20170203-14)
  • 【文献出处】 北京农业职业学院学报 ,Journal of Beijing Vocational College of Agriculture , 编辑部邮箱 ,2019年05期
  • 【分类号】S858.28
  • 【下载频次】131
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