【摘要】 目的探讨胞质多聚腺苷酸化元件结合蛋白4(CPEB4)在透明细胞性肾细胞癌(ccRCC)患者ccRCC组织中的表达情况及与患者临床特征和预后的关系。方法收集76例ccRCC患者的ccRCC组织及其中32例患者的癌旁(距肿瘤外缘≥5 cm)正常组织标本,采用实时定量聚合酶链反应(RT-PCR)和免疫组织化学染色法检测不同组织中CPEB4 mRNA和蛋白的表达情况,分析ccRCC组织中CPEB4 mRNA的阳性表达情况与ccRCC患者临床特征及预后的关系,以及ccRCC患者预后的影响因素。结果 ccRCC患者ccRCC组织中CPEB4蛋白的高表达率和CPEB4 mRNA的阳性表达率均明显高于癌旁正常组织(P﹤0.01)。不同年龄、性别、肿瘤部位的ccRCC患者ccRCC组织中CPEB4 mRNA的阳性表达率比较,差异均无统计学意义(P﹥0.05);不同TNM分期、世界卫生组织(WHO)组织学分级和淋巴结转移情况的ccRCC患者ccRCC组织中CPEB4 mRNA的阳性表达率比较,差异均有统计学意义(P﹤0.05)。Cox比例风险模型分析结果显示,TNM分期为Ⅲ～Ⅳ期和CPEB4 m RNA阳性表达均是ccRCC患者预后的独立危险因素(P﹤0.05)。CPEB4 m RNA阴性表达患者的3年生存率明显高于CPEB4mRNA阳性表达患者(P﹤0.01)。结论 TNM分期为Ⅲ～Ⅳ期和CPEB4 mRNA阳性表达均是ccRCC患者预后的独立危险因素,CPEB4的表达情况对临床ccRCC治疗方案的选择及ccRCC患者预后的评估具有重要的临床指导价值。更多还原

【Abstract】 Objective To analyze the expression of cytoplasmic polyadenylation element binding protein 4(CPEB4)in the clear cell renal cell carcinoma(ccRCC) tissues of ccRCC patients, and to investigate the relationship with patients’ clinical characteristics and prognosis. Method The ccRCC tissue from 76 patients with ccRCC and adjacent normal tissues(≥ 5 cm from the outer edge of tumor) from 32 cases out of the 76 ones were collected, subsequently, the expression of mRNA and protein of CPEB4 in different tissues were detected by real-time polymerase chain reaction(RT-PCR) and immunohistochemical assay, to determine the relationship between the positive expression of CPEB4 m RNA and patients’ clinical characteristics, besides, the factors affecting the prognosis of ccRCC were also determined. Result The high expression rate of CPEB4 proteins and the positive expression of CPEB4 mRNA in ccRCC tissues were significantly higher than adjacent normal tissues, indicating statistically significant differences(P<0.01). The positive expression rate of CPEB4 mRNA in ccRCC tissues of ccRCC patients of different ages, genders and tumor site was not statistically significant(P>0.05). The positive expression rate of CPEB4 m RNA in ccRCC tissues of ccRCC patients with different TNM stages, WHO histological grades and lymph node metastasis were statistically significant(P<0.05). Cox proportional hazard model revealed that, TNM stage was Ⅲ-Ⅳ and positive expression of CPEB4 mRNA were independent risk factors for ccRCC patients(P<0.05). The three-year survival rate of patients with negative CPEB4 mRNA expression was significantly higher than patients with positive CPEB4 m RNA expression(P<0.01). Conclusion TNM stage is Ⅲ-Ⅳ and positive expression of CPEB4 mRNA are independent risk factors for the prognosis of patients with cc RCC, furthermore,CPEB4 expression is clinically valuable for determining the therapy and evaluating prognosis for patients with ccRCC.更多还原