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pH值、灭菌条件和凝固剂对马铃薯组培苗生长的影响

Effects of pH Value, Sterilization Conditions and Jelling Agent Plantasan on Growth of Potato Plantlet

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【作者】 刘芳陈广侠孔海明马蕾杨煜郭晓杨晓慧董道峰杨元军

【Author】 Liu Fang;Chen Guangxia;Kong Haiming;Ma Lei;Yang Yu;Guo Xiao;Yang Xiaohui;Dong Daofeng;Yang Yuanjun;Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences/Shandong Key Laboratory of Greenhouse Vegetable Biology/Shandong Branch of National Vegetable Improvement Center;National Engineering Research Center for Potato/Laoling Xisen Potato Industry Group Co., Ltd.;

【通讯作者】 杨元军;

【机构】 山东省农业科学院蔬菜花卉研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心国家马铃薯工程技术研究中心/乐陵希森马铃薯产业集团有限公司

【摘要】 为改善马铃薯组培苗的生长状态,本试验以Favorita组培苗为材料,研究培养基pH值、凝固剂和灭菌条件对马铃薯植株生长的影响。结果表明,在120℃灭菌20 min的培养基上,植株生长较好。培养基pH值为5.5时,植株最高,叶片数最多。植株在5.0 g/L车前子胶的培养基上生长健壮,干物质积累量最多。车前子胶培养基的渗透压最高,其凝胶强度仅为琼脂培养基的36.14%。

【Abstract】 In order to improve the growth vigor of potato plantlet, the plantlets of cultivar. Favorita were used as materials to investigate the effects of pH value, jelling agents and sterilization conditions on the growth of potato plantlets. The results showed that the plantlets grew better on the medium sterilized at 120℃ for 20 min. The plant height and the number of leaves were higher at pH 5.5. Compared with the agar medium, the plant lets grew more robustly and the dry matter accumulation was higher on the 5.0-g/L plantasan medium. Moreover, the plantasan medium had the highest osmotic pressure, and the gel strength was only 36.14% of that of the agar medium.

【基金】 山东省农业良种工程项目“马铃薯新品种培育与示范”(2016LZGC006);山东省现代农业产业技术体系薯类产业创新团队(SDAIT-16-05)
  • 【文献出处】 山东农业科学 ,Shandong Agricultural Sciences , 编辑部邮箱 ,2019年03期
  • 【分类号】S532
  • 【被引频次】3
  • 【下载频次】314
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