【摘要】 利用MDBK细胞从奶牛卵巢中分离到一株牛传染性鼻气管炎病毒（IBRV）毒株。设计扩增IBRV的通用引物,对卵巢组织中的病毒进行PCR扩增及测序;对卵巢组织感染MDBK细胞,进行IBRV的分离培养;测定IBRV的TCID₅₀,同时利用IBRV FA进行鉴定。结果表明,分离到的病毒在MDBK细胞上产生了明显的病变;用特异性引物可扩增出特异性目的条带,目的序列与IBRV基因序列一致;该毒株的病毒滴度为10^6.75 TCID₅₀/mL。更多还原

【Abstract】 A strain of IBRV was isolated from cow ovary with MDBK cells. The universal primer of IBRV was designed and amplified, and the ovarian tissue was used for PCR and sequencing. The ovarian tissue infected MDBK cells to isolate the IBRV; the TCID₅₀ of IBRV was determined and it was identified with IBRV FA. The results showed the cytopathic effect（CPE） generated in MDBK. The specific bands could be amplified by PCR with IBRV primers. The sequencing results showed that the sequence was coincided with the published IBRV sequence. The virus titration of the strain was 10^6.75 TCID₅₀/mL.更多还原