【摘要】 以从浓香型白酒窖泥中分离得到的酪丁酸梭菌RL1(Clostridium tyrobutyricum RL1)为研究对象,采用单因素试验结合正交试验优化菌株产丁酸的发酵条件.结果表明,酪丁酸梭菌RL1的最优产丁酸发酵条件为:发酵培养基初始pH=6. 8,装液量100%,培养温度34℃,接种量3%(体积分数),还原剂为硫代乙醇酸钠且其添加量为0. 5 g/L.在该发酵条件下,酪丁酸梭菌RL1的丁酸产量可达10. 66 g/L,较优化前提高了41. 76%.更多还原

【Abstract】 The fermentation conditions of Clostridium tyrobutyricum RL1 isolated from Luzhou-flavor liquor pit mud for the production of butyric acid was optimized using one-factor experiment combined with orthogonal experiment. The results showed that the optimal yield of butyric acid termentation of Clostridium butyrium RL1 was the initial p H value 6. 8,the volume of culture media 100%,the culture temperature 34 ℃,the inoculation amount 3%( volume fraction) and the dosage of the deoxidizer( sodium thioglycolate) 0. 5 g/L. Under the optimal fermentation conditions,the production of butyric acid by Clostridion tyrobutyricum RL1 reached10. 66 g/L,which was 41. 76% higher than that before optimization.更多还原