The compositions and contents of fatty acid and unsaponifiable matter in Staphylea bumalda seed oil were analyzed by GC-MS. The results showed that the content of unsaturated fatty acid in Staphylea bumalda seed oil was 86. 68%,mainly were oleic acid,linoleic acid and linolenic acid. Six phytosterols,three olefins,one vitamin and twelve alkane compounds were identified from the unsaponifiable matter,and γ-sitosterol,squalene and vitamin E were abundant with contents of 411. 21,257. 72mg/kg and 151. 94 mg/kg...