KOH/Al-pillared bentonite catalyst was prepared by the method of incipient wetness impregnation. Biodiesel was produced by transesterification of Jatropha oil catalyzed by KOH/Al-pillared bentonite. The influences of KOH loading amount and transesterification reaction conditions on the conversion rate of biodiesel and regenerability of the catalyst were studied. KOH/Al-pillared bentonite catalyst was characterized by scanning electron microscopy( SEM) and X-ray diffraction( XRD). The results showed that the...